The knee joint has also a periarticular adipose tissue, which is known as Hoffa's fat pad (IPFP). IPFP has a dual function in the joint it reduces the concentration of Nitric Oxide, the release of glycosaminoglycans and the expression of MMP1 in the cartilage, but it also contains MSC and macrophages. Our hypothesis is that synovial fluid contains elements, not all of which are understood, which act as messengers and alter the “homeostasis” of the knee and the metabolism of all the cellular components of the joint, including the MSC of Hoffa's fat pad, thus making them another piece in the puzzle as far as OA of the knee is concerned.

The IPFP of 37 patients with OA and 36 patients with ACL rupture were analyzed. Isolation, primary culture, and a functional and proteomic study of MSCs from IPFP were performed.

Our results show that OA of the knee, in its more severe phases, also affects the MSC's of IPFP, which is a new actor in the OA degenerative process and which can contribute to the origin, onset and progression of the disease. A differential protein profile between OA and ACL patients were identified.

Infrapatellar pad should be regarded as an adipose tissue with its own characteristics and it´s also able to produce and excrete important inflammatory mediators directly into the knee joint.

Introduction: Ankle arthrodesis is still a surgical technique, which implies compensations with a higher range of motion from the neighbouring joints (knee-tarsus). The aim of this study is to compare and analyze the functional results of large outcome ankle arthrodesis through both kinetic and kinematical motion study and plantar support study.

Material and Methods: We studied 19 patients (17 males and 2 females) with post-traumatic ankle arthrodesis (15 right side and 4 left side) with a minimum follow up of three years (3–9 years). No other lower extremity pathologies were associated. Average age was 37 years (23–56 y.o) and average weight 84,5 kg (54–117 Kg).

We performed physical (including body mass index, BMI), functional and image examination (X-Rays and CT scan). In order to objectify the results we used SF-36, AOFAS scale (max 90 points) and Mazur scale (max 97 points). Kinetic parameters of motion with two force plates (Kistler, Switzerland) and pedography (Emed, Novel, Munich, Germany) were obtained. Kinematic data were obtained with a 3-D video analysis system (Clima system, STT, San Sebastian, Spain). A statistical descriptive study was performed to know the grade of patients’ satisfaction and to analyze the range of motion (ROM) and reaction force of the limbs. Both sides were compared.

Results: results obtained with Mazur scale were 49,26 points (14 – 83), 49,89 points AOFAS scale (22 – 84), 85,79 points SF-36 (85 – 109). Pain score (VAS) was 4.1 points. BMI over 30 points was found six patients. Image analysis of all patients showed an adequate fracture consolidation and a correct ankle position. Kinematical study expressed a lower ROM in the fusion ankle than in the healthy one, basically due to the neighbouring joints movement compensation. Regarding motion parameters we only found differences at the anterior-posterior force switch direction point (41,67% of the healthy foot step and 50,37% in the fusion foot step). Support time was greater in fusion ankle than in healthy one. Midfoot and centre forefoot pressures resulted greater in fusion feet.

Conclusion: Patients with ankle arthrodesis presented often overweight. They show a good subjective outcome in the quality life scales and few mechanical alterations despite of the low score of the ankle and pain specific rating scales.

Introduction: Anterior cruciate ligament (ACL) rupture leads to biomechanics disturbances of the knee joint which are reflected also in the plantar supports. Our hypothesis is that a redistribution of the sole bilateral charges will be produced to allows the feet to get a new control system to compensate ACL rupture. The aim of this research is to study the plantar support pressures disturbances in patients with ACL rupture before operation.

Material and Methods: We analyzed the plantar pressure distribution in two populations: Group A: 39 males of 37 years average age (21–49 y.o), previous surgery of isolated ACL rupture, excluding patients with meniscal tear or serious cartilage damage, contralateral lesions and knee previous surgery as well. Group B (control group): 37 healthy males of 31 years average age (21–40 y.o) without any musculoskeletal disorders.

We performed physical examination and walking through a pedography plate (Emed, Novel Munich, Germany). We studied global plantar support (pressure, forces and areas) of each foot and also divided each foot into six parts. Data obtained was compared between group A, patients (healthy leg and ACL rupture leg) and group B (control group). Statistical analysis was performed with a non-parametric Wilcoxon test.

Results: Group A (healthy leg and ACL rupture leg) total support area of both feet were statistically superior than Group B total support area (p< 0,019 and p< 0,005 respectively). Evenly midfoot total support area was superior in Group A that in Group B, as well as midfoot force support (p< 0.089).

Group A midfoot pressure was higher in ACL rupture leg than in healthy leg (p< 0.007) and it was also higher to the one obtained for group B (p< 0.046). Evenly the anterior-external region of Group A, healthy leg got the highest pressure (p< 0.076), followed by Group A, ACL rupture leg (p< 0.022) and finally Group B.

Group B anterior-internal pressure was statistically superior to Group A, ACL rupture leg (p< 0.049) followed by Group A, healthy leg (p=0.022). During foot takeoff, first toe pressures were higher in Group B compared to Group A (p< 0.076).

Conclusion: ACL rupture shows differences in plantar support pressures distribution of both legs (ACL rupture leg and healthy leg) compared with a control population. The injured leg seeks balance decreasing heel support and increasing the contact surfaces between floor, midfoot and forefoot.

Objective: to study the surgical applicability and clinical results of the Vira^® system in treatment for severe fractures of the calcaneus.

Material and Method: 50 acute intraarticular fractures of the calcaneus treated with the Vira^® system over a two-year period were analyzed in 42 patients. The mean age was 41 years. Eight were bilateral fractures and 47 were closed fractures. According to the Sanders classification, most of the fractures were type IV, followed by type III AB, and the inferior cortical was broken in 42 cases. All the patients were evaluated, in a prospective manner, using the AOFAS scale, plain radiographs and CT scan studies.

Results: the average AOFAS score 12 months after surgery was 76.6 points (SD: 13.9). In 26% cases the results were very good, in 62% good, and in 12% mild and poor. The Böhler angle improvement after surgery was significant (p=0.05) though clinically irrelevant. Subtalar arthrodesis was achieved in all but two cases. The post-surgical complications were: 4 disorders of the plantar support, 4 cases of osteolysis at the tip of the screws, and 3 soft tissue problems.

Conclusions: The Vira system is a validated option for the surgical treatment of severe fractures of the calcaneus, yielding good clinical and radiological results with a surgical procedure that is only minimally aggressive and has a low rate of complications.

Aim: Total Wrist fusion is the main procedure for treatment of postraumatic and degenerative wrist osteoarthritis. During the last decade, midcarpal fusion has become more and more popular as it preserves motion. The purpose of this study was to compare the functional and workers compensation results of both treatments on active workers.

Material and Methods: A prospective study to evaluate 77 patients (76 male and 1 female) who underwent wrist fusion, between 2002 to 2006, with an average of 28 months of follow-up (range, 12–58 months) were made. The mean age were 32 years (range, 25–48 years) and all patients were medium or high level workers with postraumatic and degenerative wrist osteoarthritis. The aethiology in 67% of the patients was SNAC wrist. Right hand was involved in 65% of the patients.

Thirty-eight patients were treated with scaphoid excision and 4-corner fusion using dorsal circular plate. Thirty-nine patients were treated with total wrist fusion using one single, dorsal, precontoured and tapered plate for osteosynthesis and third carpometacarpal joint (CMCJ-3) was included. All patients were immobilised in a cast for 4 weeks after surgery.

Postoperative complications, pain (visual analogue scale), clinical and functional outcome based on Green and O’Brien score, grip strength, X-ray evaluation, time to return to work and activity level were evaluated and compared.

Results: Consolidation was obtained in all cases at 10 weeks. Pain evaluation score was 19,2 (4-corner) and 13,8 (total fusion) on post-op time. The mean modified Mayo wrist score was 70,4 (4-corner) and 69 (total wrist). Average lost of pinch strength was 43% (4corner) and 21% (total fusion). 2 patients with 4-corner fusion required total wrist arthrodesis. Three cases who had a total wrist fusion, required implant removal.

The average time to return to work was 17 weeks (4-corner) and 16,2 weeks (total fusion). All patients return to work. Twelve percent of four-corner fusion and 72% of total wrist fusion return to the same work level with restrictions (until 33% of activity). Twenty-two percent of 4-corner fusion and 28% of total wrist fusion were unable to return to their previous activity level, performing lower intensity work activities. Overall satisfaction was high in both groups with 85% (4corner) and 93% (total fusion).

Conclusion: Both fusion techniques allows an effective stabilization, maintaining the bone stock and eliminate wrist pain with fast return to work.

Total wrist fusion had less surgical failures, better level of satisfaction, lesser lost of force than 4-corner fusion, with less potential for further deterioration with time. However, 4-corner fusion allows return to work with a similar activity level and preserve a functional range of motion in patients with high levels of activity.

Introduction and purpose: The purpose of this study was to assess the functional results of ankle arthrodesis as a treatment of posttraumatic sequelae.

Materials and methods: We carried out a retrospective study from the 1st of January 2000 to the 31st of December of 2005 with a total of 55 patients that underwent posttraumatic ankle arthrodesis, with a mean follow-up of 5 years (range: 1–7 years). The mean age of the patients was 40 years (range: 18–61). There were 52 men and 3 women, the right side (40) predominated over the left (15). Seventy-eight percent of trauma sustained was high-energy with a predominance of fracture of the tibial pilon (type C3 fractures on the AO classification). There were 35% open fractures. The predominant type of surgical technique performed used cannulated screws without any iliac crest grafts. The mean time from surgery to discharge was 9 months (range 4–19).

Results: Repeat arthrodesis was necessary in 16% and an infrapatellar amputation was necessary in 1 case. Only 1 patient returned to their workplace without limitations, the rest were discharged to a medical board for sequelae. The use of crutches/insoles and subjective assessment of pain were related to a lateral tibiotalar angle greater than 90° and varus deformity.

Conclusions: Tibiotalar arthrodesis is a useful rescue technique in patients with severe posttraumatic ankle sequelae. It eliminates pain and increases stability but is the cause of a significant loss of function of the lower limb.

Introduction and purpose: The VIRA system allows reconstruction of the fractured bone and its arthrodesis with the talar bone eliminating all movement of the subtalar joint by means of a minimally invasive approach. The aim is to restore the shape of the foot and prevent joint degenerative changes in the long term without the complications and sequelae of open surgery.

Material and methods: Between November 2004 and June 2006, 30 intraarticular calcaneal fractures in 25 patients were treated surgically by means of the Vira System. Using Sanders’ classification 6 cases were classified as type II, 13 as type III and 11 as type IV. Five patients had open fractures. There was a mean period of 8.7 days between the accident and surgery. Seven cases had associated lesions and fractures in other locations. Only 2 cases required iliac crest grafts in the operated area.

Results: All patients except 10 have returned to their usual occupations. Eight cases evolved favorably but have had a short follow-up. Two cases experienced a delayed healing of the subtalar arthrodesis and had to be reoperated; they required an autologous graft. The mean period of temporary disability in patients discharged from hospital was 163.7 days. Clinical assessment using the AOFAS scale reached a mean value of 76.6 points. No surgical or post-surgical complications were seen in the group studied.

Conclusions: The VIRA System seems capable of achieving its purpose in the first series of patients operated. It allows a quick recovery both postoperatively and in the subsequent evolution without any associated complications. It accelerates the patients’ return to work by decreasing convalescence and minimizing sequelae.

Introduction: Loss of the meniscus frequently leads to progressive arthritic changes in the involved compartment. Replacement of the lost meniscus with a resorbable collagen scaffold has been proved to allow regeneration of meniscal tissue.

MATERIAL AND Methods: Between June 2001 and September 2004, 42 patients, 38 male and 4 female, underwent arthroscopic placement of a collagen meniscus implant (CMI) to reconstruct the damaged medial meniscus. Their age ranged between 22 and 50 years. All patients had an associated ACL insufficiency, 39 secondary to acute ACL tear and 3 ACL graft failures. ACL reconstruction with hamstrings grafts (39) and ACL revision with allograft (3) was performed at the time of CMI implantation. The interval between ACL injury and surgery ranged between 3 weeks and 6 months. Ten patients also had a lateral meniscus tear. All patients were followed with clinical, weight-bearing radiographs, KT-1000 and magnetic resonance examinations for at least 18 months (range, 18– 84 months). The IKDC form was used to record and evaluate the Results:

Results: The length of the implanted CMI ranged between 3 and 5.5 cm and required 4 to 8 stiches.

The IKDC subjective evaluation was normal in 18 patients, nearly normal in 18, abnormal in 5 and severely abnormal in 1. Range of motion was normal in 28 patients and nearly normal in 14. KT 1000 examination was normal in 32 patients, nearly normal in 7, abnormal in 1 and severely abnormal in 2.

The X ray findings were normal in 28 patients, nearly normal in 6 and abnormal in 8.

Complications included 2 saphenous nerve neuritis, 1 ACL graft tear with CMI implant breakage and 2 knee stiffness that required mobilization.

40 patients returned to work. The average time to resume work was 5.5 months

Conclusion: Simultaneous ACL reconstruction and collagen meniscal implantation is a viable and effective option in young active patients with ACL insufficiency and associated meniscal injuries.

Introduction: Intraarticular fractures of distal radius are associated with high energy mechanisms, are severe and difficult to obtain a surgical reduction. The aim of our paper is to compare the clinical, radiographically and activity results in workers treated with surgical and conservative procedures.

MATERIAL AND METHODS: A prospective study was organized in 43 heavy-labour workers (34 male and 9 female) with unstable fractures of distal radius, treated between 2003 and 2005. The minimal follow-up was of one year. The mean age were 40 years (22–65 years) and dominant limb was involved in 40% of the cases. To treatment groups were established. Group 1, conservative treated with indirect reduction and cast immobilization (n=20) and Group 2 surgically treated with indirect reduction and percutaneous fixation with K-wires and cast immobilization (n=24).

Pain, mobility and radiograhs were evaluated and also strength (isokinetics), functionality (DASH score) and, finally, the return to work at 3, 6 and 12 months.

RESULTS: Fracture healing was obtained at 7 weeks but the time to return to work were 14 weeks after surgery. Pain score, at 12 months, were 2,3 points for conservative treatment and 2,9 points for the surgically group. The flexion – extension mobility loss, in relation to the contralateral wrist, was lesser in the conservative group at 3 and 6 month but similar (11°) at 12 months in both groups. Radiographs corrections were anatomically in 38% of the cases of group 1 and in 80% of group 2. Functional and strength results were similar in both groups. All the patients return to the same work activity.

CONCLUSIONS: Percutaneous fixation of unstable intraarticular distal radius fracture is comparable to the conservative treatment but the percentage of anatomical reductions was higher. It would be of importance in the evolution of the patients.

AIM: We present the results of scaphoid non-unions treated with open reduction, bone grafting and internal fixation with biodegradable implants on active heavy labour workers.

MATERIAL AND METHODS: Between 2002 to 2004, 20 patients with scaphoid non-unions were treated by open reduction, bone grafting and internal fixation using self-reinforced poly-L-lactic acid screws. The mean prospective follow-up was 24 months (range, 12–38 months). The mean age was 28 years (range, 18–42 years). All patients were male and heavy-labour workers.

The patients were assessed clinically (modified Mayo wrist score) and radiograhically. The grip and pinch strength were also studied.

RESULTS: We find 13 excellent results, good in 5 cases and poor in 2 cases. A Matti-Russe group patient was revised 6 months after the first intervention. Any fragment displacement, implant loosening or adverse reaction was found. Most of the patients (90%) return to the same work and the same level without complications.

CONCLUSION: Both groups are a good alternative for the scaphoid non-unions treatment. However, biodegradable implants disappears in the time, the removal are no necessary, facilitate the revision surgery, if necessary, and permitted MRI studies to evaluated the graft viability.

The aim of this study was to analyse the morphological differences of the intervertebral disc at different levels focusing in the endplate and the anchorage of the disc fibres to the vertebrae and the distribution pattern of collagen I and II.

This study was conducted on 45 intervertebral discs from nine monkeys (Macaca fascicularis). All slices were processed for histological, histomorphometrical and immunohistochemical analysis.

The endplate was formed, at all the levels, by 3 zones: a cartilaginous zone adjacent to the nucleus pulposus, an intermediate mineralised zone of cartilage and a growth cartilaginous zone adjacent to the vertebrae.

The inner annular fibres anchored to the not mineralised cartilaginous endplate zone, whereas the outer annular fibres anchored to the mineralised cartilaginous endplate zone.

The height of the intervertebral disc varied along the length of the spine. The smallest value was measured in T3–T4, with a larger increasing caudally than cranially. The highest value was measured in L2–L3. A cervical intervertebral disc was the 55% of a lumbar one.

The findings of this study provide a detailed structural characterization of the IVD and may be useful for further investigations on the disc degeneration process.

Introduction and purpose: We have studied the in-vitro response of older and osteoarthritic chondrocytes when confronted with various factors in order to analyze the possible reversion of their phenotype to that of healthy chondrocytes.

Materials and methods: The study used cartilage from young (3 months’ old) and old (7 years old) lambs with an osteoarthritic pathology. The latter group was obtained by means of a meniscectomy after a two-month evolution. Cells coming from the femur cartilage were isolated by means of collagenase digestion and cultured in a single layer using a DMEM culture medium supplemented with 10% fetal serum, penicilin and streptomycin, hepes and L-cystein (Gibco-BRL®). BrdU incorporation assays were performed by means of an ELISA protocol in order to analyze the proliferation rate. Later, a gene expression analysis was conducted using RT-PCR. The treatment was carried out at a concentration of 50 ng/mL using FGFa, IGF-a, TGF-b (Peprotech Inc) and OP-1 (Stryker) growth factors, and later growth factor combinations FGFa/IGF-1, FGFa/OP-1, FGFa/TGF-b and TGF-b/OP-1.

Results and conclusions: Comparison between the three groups showed that the proliferation rate was lower in older and osteoarthritic cells. These last two groups, however, did not have the same expression pattern as the genes studied. The analysis of the response to growth factors showed that FGFa and IGF-1 were the most efficient ones, and their combination proved to be the most powerful of all.