Advertisement for orthosearch.org.uk
Results 1 - 3 of 3
Results per page:
Applied filters
Content I can access

Include Proceedings
Dates
Year From

Year To
Bone & Joint Research
Vol. 10, Issue 10 | Pages 693 - 703
1 Oct 2021
Wang X Wang D Xia P Cheng K Wang Q Wang X Lin Q Song J Chen A Li X

Aims

To evaluate the effect of ultrasound-targeted simvastatin-loaded microbubble destruction (UTMDSV) for alleviation of the progression of osteoarthritis (OA) in rabbits through modulation of the peroxisome proliferator-activated receptor (PPARγ).

Methods

In vitro, OA chondrocytes were treated with ultrasound (US), US-targeted microbubble destruction (UTMD), simvastatin (SV), and UTMDSV on alternate days for four weeks. Chondrocytes were also treated with PPARγ inhibitor, PPARγ inhibitor+ UTMDSV, and UTMDSV. The cholesterol efflux rate and triglyceride levels were measured using an assay kit and oil red O staining, respectively. In vivo, the OA rabbits were treated with a single intra-articular injection of UTMD, SV, and UTMDSV every seven days for four weeks. Cartilage histopathology was assessed by safranin-O staining and the Mankin score. Total cholesterol (TC) and high-density lipoprotein-cholesterol (HDL-C) in rabbit knee synovial fluid were detected by enzyme-marker assay. Aggrecan, collagen II, and PPARγ expression levels were analyzed by Western blotting (WB).


Orthopaedic Proceedings
Vol. 93-B, Issue SUPP_III | Pages 275 - 276
1 Jul 2011
Gandhi R Razak F Tso P Davey JR Mahomed NN
Full Access

Purpose: Metabolic syndrome (MS) is defined as central adiposity, elevated fasting glucose, hypertension, and dyslipidemia defined as high triglyceride and low high-density lipoprotein (HDL) cholesterol. MS is associated with a systemic proinflammatory and prothrombotic state. We asked if patients with MS undergoing total knee arthroplasty (TKA) have an increased risk for symptomatic deep vein thrombosis at 3 months follow-up (DVT). Method: 1,460 consecutive patients were reviewed from our joint registry undergoing primary, unilateral TKA between the years of 1998–2006. Demographic variables of age, gender, comorbidity, and education were retrieved. Metabolic syndrome (MS) was defined as body mass index above 30 kg/m2, diabetes, hypertension, and hypercholesterolemia. Logistic regression was used to examine the relationship of MS on the incidence of DVT. Results: The overall incidence of symptomatic DVT was 4.4% (65/1460). Patients with MS had an increased incidence of DVT as compared to those without MS(15.5% vs 3.4%). Adjusted analysis showed that MS increased the risk of symptomatic DVT by 3.2(95% CI [1.0,15.4], p=0.04) times compared to those without MS. Conclusion: Hospital protocols developed for prophylactic anti-coagulation following TKR should give special consideration to patients with MS


Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_VIII | Pages 27 - 27
1 Mar 2012
Ikemura S Yamamoto T Nishida K Motomura G Iwamoto Y
Full Access

Introduction. The objective of this study was to investigate the incidence of steroid-induced osteonecrosis (ON) among male and female rabbits. Methods. Forty-seven adult rabbits (male, n = 24; female, n = 23) were injected once intramuscularly into the right gluteus medius muscle with 20 mg/kg of methylprednisolone acetate. Hematological examinations were performed just before and at 1 and 2 weeks after the corticosteroid injection. Two weeks after the injection, both femora and humeri were histopathologically examined for the presence of ON, and the bone marrow fat cells were examined morphologically. Results. Sixteen of 24 male rabbits (66.7%) developed ON, with only 5 of 23 female rabbits (21.7%) developing ON. There was a significant difference in the rate of incidence of osteonecrosis between male and female rabbits (p = 0.0032). Hematologically, at 1 week after the corticosteroid injection, both the mean low-density lipoprotein (LDL) and the ratio of LDL cholesterol to high-density lipoprotein (HDL) cholesterol in the male rabbits were significantly higher than those in the female rabbits (p = 0.0001 for both comparisons). The bone marrow fat cells of the male rabbits (61.5 +- 5.6 μm) were significantly larger than those of the female rabbits (58.9 +- 3.7 μm) (p = 0.0102). Conclusion. This study indicates that gender may be an important factor in considering the pathogenesis of corticosteroid-induced ON