Objectives. In order to screen the altered gene expression profile in peripheral blood mononuclear cells of patients with osteoporosis, we performed an
Aims. This study aimed to uncover the hub long non-coding RNAs (lncRNAs) differentially expressed in osteoarthritis (OA) cartilage using an
Aim. Osteoarthritis (OA) is caused by complex interactions between genetic and environmental factors. Epigenetic mechanisms control the expression of genes and are likely to regulate the OA transcriptome. We performed integrative genomic analyses to define methylation-gene expression relationships in osteoarthritic cartilage. Patients and Methods. Genome-wide DNA methylation profiling of articular cartilage from five patients with OA of the knee and five healthy controls was conducted using the Illumina Infinium HumanMethylation450 BeadChip (Illumina, San Diego, California). Other independent genome-wide mRNA expression profiles of articular cartilage from three patients with OA and three healthy controls were obtained from the Gene Expression Omnibus (GEO) database. Integrative pathway enrichment analysis of DNA methylation and mRNA expression profiles was performed using
Robotic-assisted total knee arthroplasty (TKA) has proven higher accuracy, fewer alignment outliers, and improved short-term clinical outcomes when compared to conventional TKA. However, evidence of cost-effectiveness and individual superiority of one system over another is the subject of further research. Despite its growing adoption rate, published results are still limited and comparative studies are scarce. This review compares characteristics and performance of five currently available systems, focusing on the information and feedback each system provides to the surgeon, what the systems allow the surgeon to modify during the operation, and how each system then aids execution of the surgical plan. Cite this article: Abstract
Aims: The choice of the treatment of tibial plateau fractures remains a controversial topic in current traumatology practice. The best treatment must have three fundamental requirements: to be less invasive as possible, to result in a good reduction, to have a good stability. Surgical approach with percutaneous indirect articular reduction by elevating,minimal osteosynthesis and the use of NORIAN Skeletal Repair System lets us reach three fundamental objectives stated before. NORIAN S.R.S, used to fill the bone gap resulting from the traumatic collapse of the metaphyseal bone, with its mechanical strength allows the stabilization of the joint fragments, the reduction and the relative ostheosyntesis, thus greatly shortening the functional recovery time. Methods: We reviewed 70 patients affected by tibial plateau fractures, treated with this percutaneous technique using the mineral bone substitute 56 fractures were uni-condylar, 21 type 41-B2 and 35 type 41-B3 (according to AO/OTA classificaction); 14 fractures were bicondylar (AO/OTA 41-C3). The minimum follow-up was 1 year. We used for clinical evaluation the Hohl assessment form, for the radiographs the criteria of Rasmussen. X-rays. Results: The final conclusions, resulting from
The choice of the treatment of tibial plateau fractures remains a controversial topic in current traumatology practice. The best treatment must have three fundamental requirements: to be less invasive as possible, to result in a good reduction, to have a good stability. Surgical approach with percutaneous indirect articular reduction by elevating,minimal osteosynthesis and the use of NORIAN Skeletal Repair System lets us reach three fundamental objectives stated before. NORIAN S.R.S, used to fill the bone gap resulting from the traumatic collapse of the metaphyseal bone, with its mechanical strength allows the stabilization of the joint fragments, the reduction and the relative ostheosyntesis, thus greatly shortening the functional recovery time. We reviewed 70 patients affected by tibial plateau fractures, treated with this percutaneous technique using the mineral bone substitute Norian:. 56 fractures were unicondylar, 21 type 41-B2 and 35 type 41-B3 (according to AO/OTA classificaction); 14 fractures were bicondylar (AO/OTA 41-C3). The minimum follow-up was 1 year. We used for clinical evaluation the Hohl assessment form, for the radiographs the criteria of Rasmussen. X-rays. The final conclusions, resulting from
The treatment of the complex tibial plateau fractures is often hard because in these fractures, the entity of the articular damage is always important, the reduction is not easy it entails often extensive exposure and the collapse of the metaphyseal bone, located beneath the reconstructed articular surface, makes the osteosynthesis mechanically unsafe for ten or twelve weeks. The percutaneus surgical treatment, let us reach three fundamental objectives: to be mini-invasive, to result in a good reduction, to have a stable fixation. Through a small skin incision at the metaphysis, a leever was inserted across a small door made on the cortical metaphiseal bone and the articular fragments were elevated and held in the reduced position. Temporarily the fragments were fixed with a Kirshner-wire and definitely fixed with one or more canulated screw NORIAN S.R.S, used to fill the bone gap resulted from the traumatic collapse of the metaphyseal bone, with its initial mechanical strenghth allows to stabilize the joint fragments reduction and the relative ostheosyntesis, thus shortening the functional recovery time. Since 1997 we operated 52 patients affected by tibial plateau fractures using this percutaneus technique with the application of the mineral bone substitute Norian. 42 fractures were unicondylar: 16 type B2 and 26 Type B3 according to AO classificaction; 10 fractures were bicondylar 4 type C2 and 6 type C3 AO classificaction. The minimum follow-up was 1 year. We used for clinical evaluation the Hohl assessment form, for the radiographs the criteria of Rasmussen X-rays. The final conclusions, resulting from
The treatment of complex tibial plateau fractures is often difficult because in these fractures the nature of the articular damage is always important, the reduction is not easy, often entailing extensive exposure, and the collapse of the metaphyseal bone, located beneath the reconstructed articular surface, makes the osteosynthesis mechanically unsafe for 10 or 12 weeks. There are three fundamental objectives of percutaneous surgical treatment: to be minimally invasive, to result in a good reduction and to achieve stable fixation. Through a small skin incision at the metaphysis, a leever was inserted across a small door made on the cortical metaphyseal bone and the articular fragments were elevated and held in the reduced position. Temporarily the fragments were fixed with a Kirschner wire and definitely fixed with one or more cannulated screws. Norian S.R.S was used to fill the bone gap that resulted from the traumatic collapse of the metaphyseal bone: with its initial mechanical strength the joint fragment reduction and the developing ostheosynthesis can be stabilised, thus shortening the functional recovery time. Between 1997 and 2002 we operated 52 patients with tibial plateau fractures using this percutaneous technique and application of the mineral bone substitute Norian. Of these, 42 fractures were unicondylar, 16 type B2 and 26 Type B3 according to the AO classification, and 10 fractures bicondylar 4 type C2 and 6 type C3 according to the AO classification. The minimum follow-up was 1 year. For clinical evaluation we used the Hohl assessment form, for the radiographs the criteria of Rasmussen X-rays. The final conclusions, resulting from
Introduction. The prevalence of symptomatic osteoarthritis (OA) in the knee is 11–11% compared to 3.4–4.4% in the ankle. In addition to this, 70% of ankle arthritis is post-traumatic while the vast majority of knee arthritis is primary OA. Several reports have previously implicated biochemical differences in extracellular matrix composition between these joint cartilages; however, it is unknown whether there is an inherent difference in their transcriptome and how this might affect their respective functionality under load, inflammatory environment etc. Therefore, we have analysed the transcriptome of ankle and knee cartilage chondrocytes to determine whether this could account for the lower prevalence and altered aetiology of ankle OA. Methods. Human full-depth articular cartilage was taken from the talar domes (n=5) and the femoral condyles (n=5) following surgical amputation. RNA was extracted and next generation sequencing (NGS) performed using the NextSeq®500 system. Statistical analysis was performed to identify differentially regulated genes (p adj < 0.05). Data was analysed using
The aim of this study was to identify key pathological genes in osteoarthritis (OA). We searched and downloaded mRNA expression data from the Gene Expression Omnibus database to identify differentially expressed genes (DEGs) of joint synovial tissues from OA and normal individuals. Gene Ontology (GO) and Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway analyses were used to assess the function of identified DEGs. The protein-protein interaction (PPI) network and transcriptional factors (TFs) regulatory network were used to further explore the function of identified DEGs. The quantitative real-time polymerase chain reaction (qRT-PCR) was applied to validate the result of bioinformatics analysis. Electronic validation was performed to verify the expression of selected DEGs. The diagnosis value of identified DEGs was accessed by receiver operating characteristic (ROC) analysis.Objectives
Methods
