Myokine developmental endothelial locus-1 (DEL-1) has been documented to alleviate inflammation and endoplasmic reticulum (ER) stress in various cell types. However, the effects of DEL-1 on inflammation, ER stress, and apoptosis in tenocytes remain unclear. Human primary tenocytes were cultured in palmitate (400 μM) and palmitate plus DEL-1 (0 to 2 μg/ml) conditions for 24 hours. The expression levels of ER stress markers and cleaved caspase 3, as well as phosphorylated 5' adenosine monophosphate-activated protein kinase (AMPK) and autophagy markers, were assessed by Western blotting. Autophagosome formation was measured by staining with monodansylcadaverine, and apoptosis was determined by cell viability assay and caspase 3 activity assay.Aims
Methods
The purpose of this study was to evaluate the mid-term clinical and radiological results in patients who were managed by double metal augmentations in proximal tibial uncontained bony defects undergoing primary or revision total knee arthroplasty We performed double metal augmentations in proximal tibial uncontained bony defects undergoing total knee arthroplasty. Out of total 14 patients, 8 patients (4 priamry arthroplasty, 4 revision arthroplasty), mean 61.3 (50–80) years, were available for review at least 5 years follow up. The average follow up period was 86.3(60–99) months. Range of motion, American Knee Society Score were evaluated pre- and postoperatively as a clinical values. Another clinical assessments undertaken at the final reviews, Western Ontario and McMaster Universities osteoarthritis index (WOMAC), Oxford knee score (OKS), Short Form-36 (SF-36), Lower extremity functional scale (LEFS), and Lower extremity activity scale (LEAS) were checked. Radiological results, involving presence of radiolucent lines (RLLs) > 1mm in width, and osteolysis at the block-cement-bone interface were taken under fluoroscopic images at postoperatively and annually thereafter.Introduction
Materials and Methods
Although minimally invasive plate osteosynthesis (MIPO) has become popular option for humeral shaft fractures, indirect reduction and its maintenance are technically challenging. The purpose of this study is to describe a reproducible technique utilizing an external fixator during MIPO and to assess its outcomes. Twenty-nine cases with a mean age of 37.1 years were included. There were 7 simple (type A) and 22 comminuted (type B or C) fractures. Indirect reduction was achieved and maintained by a monolateral external fixator on the lateral aspect of humeral shaft, and MIPO was performed on the anterior surface. Union, alignment, complications, and functional results of the shoulder and elbow were assessed.Background
Methods
To establish relative fixation strengths of a single lateral locking plate, a double locking plate, and a tibial nail in treatment of proximal tibial extra-articular fractures. Three groups of composite tibial synthetic bones consisting of 5 specimens per group were included; lateral plating (LP) using a LCP-PLT (locking compression plate-proximal lateral tibia), double plating (DP) using a LCP-PLT and a LCP-MPT (locking compression plate-medial proximal tibia), and intramedullary nailing (IN) using an ETN (expert tibial nail). To simulate a comminuted fracture model, a gap osteotomy measuring 1 cm was cut 8 cm below the joint. For each tibia, a minimal preload of 100N was applied before loading to failure. A vertical load was applied at 25mm/min until tibia failure.Background
Methods
Although gradual bone transport may provide a large-diameter bone, complications are common with the long duration of external fixation. To reduce such complications, a new technique of bone transport with a locking plate has been done for tibial bone defect. In 13 patients (mean age, 38.9 years) of chronic osteomyelitis or traumatic bone defect, segmental transport was done using external fixator with a locking plate. In surgical technique, a locking plate was fixed submuscularly, holding the proximal and distal segments. Then, the external fixator for transport was fixed without contact of the locking plate. After docking, 2 or 3 screws were fixed at the transported segment through the plate holes. At the same time, the external fixator was removed.Background
Methods
