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Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XL | Pages 112 - 112
1 Sep 2012
In Vitro Antibiotic Susceptibility of Staphylococcus Aureus and Staphylococcus Epidermidis Isolated From Retrieved Prosthetic Implants
Molina-Manso D del Prado G Manrubia-Cobo M Ortiz-Pérez A Cordero-Ampuero J Gómez-Barrena E
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INTRODUCTION

Prosthetic joint related-infections (PJRI) are severe complications in orthopaedic surgery. Staphylococcus aureus and Staphylococcus epidermidis are the most commonly isolated pathogens from implants (1). The variable antimicrobial susceptibility found in these microorganisms, makes it necessary to perform individual susceptibility studies in order to select the best antibiotic combination for clinical management (2).

MATERIAL AND METHODS

35 staphylococcal strains (17 S. aureus, 18 S. epidermidis) were isolated from PJRI using a previously described sonication protocol (3). Biofilm-producing collection strains S. aureus 15981 (4) and S. epidermidis ATCC 35984 were also included in the study. In vitro susceptibility was evaluated against 7 antimicrobial agents: rifampin, vancomycin, ciprofloxacin, cotrimoxazole, cloxacillin, clindamycin, and daptomycin. Minimal Inhibitory Concentration (MIC) assays were determined according to EUCAST recommendations and breakpoints (5). Minimal Bactericidal Concentration (MBC) was also calculated by colony counting after plating the well contents.