Advertisement for orthosearch.org.uk
Results 1 - 1 of 1
Results per page:
Applied filters
Content I can access

Include Proceedings
Dates
Year From

Year To
Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_16 | Pages 77 - 77
1 Apr 2013
Inoue M Noda T Furumatsu T Shimamura Y Kunisada T Yamakawa Y Kinami Y
Full Access

Introduction

The treatment of large segmental defects of long bones, which is caused by high energy trauma, revision surgery and resection of tumor or osteomyelitis and so on, is usually difficult. Recently the usefulness of Induced membrane technique (Masquelet technique) is reported. Induced membrane technique is an alternative method to reconstruct long-bone defects, which is two-stage surgery and combines the use of induced pseudo-membranes and cancellous autografts. The mechanism of bone formation in this technique is unknown. We performed Induced membrane technique on four patients, collected their membranes, and tested osteogenic ability and multipotency of cells derived from the membrane.

Material and Method

From 2011, we had 4 patients of large segmental defects of long bones, which underwent operations of induced membrane technique. All operation performed by one surgeon. There were 2 nonunion (2 femur) and 1 open fracture (tibia), and close comminuted fracture (femur). Average length of bone defects was 5 cm. On the second stage, we collect some membranes and cultured cells derived from them. Then, cultured cells were tested for the ability to differentiate in vitro to multiple lineage.