Abstract
Background
The main reasons for hip prosthesis failure are aseptic loosening and periprosthetic joint infection (PJI). The real frequency of PJI is probably largely underestimated because of non-standardized definition criteria, diagnostic procedure, treatment algorithm and other confounders. Therefore, data from joint registries are not reflecting the frequency of PJI and can be misleading; particularly low-grade PJI can be frequently misdiagnosed as aseptic failure. Therefore, prospective clinical studies with standardized protocol, comprehensive diagnostic procedure and sufficient follow-up should be performed. Sonication of explanted prosthesis is highly sensitive for detection of biofilms on prosthetic surface and allows quantitative analysis of biofilm formation. We hypothesize that by using sonication, ceramic components (BIOLOX®delta, BIOLOX®forte) will show higher resistance against biofilm adhesion compared to polyethylene (PE) and metal (CoCrMo).
Methods
In this prospective multicentre study (level of evidence: Ia), we included all consecutive adults ≥18 years of age, who underwent explantation of the hip prosthesis for infection or aseptic reason. Excluded were patients in whom part of the prosthetic components were retained. A standardized and comprehensive diagnostic algorithm was applied, including sonication of all removed prosthetic components for qualitative and quantitative microbiological analysis (ultrasound bath 40 kHz, 1 W/cm2, 1 min). Individual components (metal, PE, ceramic) were separately placed in sterile boxes for investigation. All patients were simultaneously included in the European Prosthetic joint infection cohort (EPJIC, www.epjic.org) to ensure long-term follow-up.
Results
Up to date, 79 patients were included, of whom 47 (60%) were diagnosed with aseptic failure and 32 (40%) with PJI. Mean age was 73 years (27–87 years), 32 (41%) were males. Table 1 summarizes the demographic characteristics. In 32 patients with PJI, most frequently isolated organisms were coagulase-negative staphylococci (n=12, 38%), Staphylococcus aureus (n=7, 22%) and Propionibacterium acnes (n=4, 13%), followed by enterococci (n=2; 6%) and gram-negative bacilli (n=2; 6%); 2 infections (6%) were polymicrobial and 3 were culture-negative (9%). Table 2 summarizes the microbiological results from sonication of removed components. Causative microorganism could be detected in sonication fluid from polyethylene in 100%, from metal in 92% and from ceramic in 69%. Significantly lower bacterial counts expressed as colony-forming units (CFU) were detected in sonication fluid from ceramic components (230 CFU/ml) than from PE (6’250 CFU/ml) and metal components (5’870 CFU / ml) (p < 0.01).
Conclusions
These first results support the hypothesis that significantly less biofilm biomass is formed on ceramic surface, compared to PE and metal surfaces, potentially indicating higher ceramic “resistance” against bacterial adhesion. These findings should be confirmed with non-microbiological investigation such as imaging (fluorescent in situ hybridization, confocal laser scanning or electron microscopy). Furthermore, in 6 of 32 patients (19%) with PJI, an aseptic loosening was preoperatively suspected. Infection was found only by systematic application of an optimized diagnostic method, particularly sonication of the removed implant. Final study results are expected to be available by the end of 2016.
