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General Orthopaedics

Effect of gamma irradiation and freeze drying on the maximum tensile strength of the cortical demineralised bone matrix

British Orthopaedic Association 2012 Annual Congress



Abstract

Introduction

Demineralised Bone Matrix (DBM) is widely used in Orthopaedics and dentistry as a bone graft substitute and may be used to augment bone formation in load bearing applications.

In this study we examine the effect of gamma irradiation and freeze drying on the tensile strength of Demineralised Cortical Bone (DCB).

Methods

Tibias were harvested from mature ewes and cut into bony strips. Demineralisation was done using 0.6M HCL and confirmed by X-ray. Specimens were washed until a pH of 7.0 +/_ 0.2 was achieved in the washing solutions.

Specimens were allocated into 4 groups; group (A) non freeze dried non gamma irradiated, group (B) freeze dried non gamma irradiated, group (C) non freeze dried gamma irradiated mention the level of gamma irradiation and group (D) freeze dried and gamma irradiated. The maximum tensile force and stress were measured. Statistical analysis using the Mann-Whitney U test was carried out.

Results

The Median of maximum tensile force for group (A) was 218N, group (B) was 306N, group (C) was 263N and for group (D) was 676N.

Group (D) results were statistically higher (p=< 0.05) compared to group (A) and (C), while there was no statistical significance compared to group (B).

Conclusion

Previously published studies suggested the possibility of using DCB as ACL graft substitute. We examined the effect of gamma radiation as the most common sterilisation technique in medical field and the freeze drying as a possible technique for long term storage on the tensile strength of the DCB.

Freeze drying significantly increases the tensile strength of the DCB while gamma irradiation has no significant effect. Our results indicate that freeze dried gamma irradiated DCB can be used as a ligament substitute.