Abstract
Development of artificial cartilage has been one of the future goals in the field of orthopaedic surgery. A few investigators have applied polyvinyl-alcohol hydrogel (single-network) to develop the artificial cartilage. However, it could not be applicable for clinical use due to insufficiency of the strength, the toughness, and the friction properties. The authors have conducted a fundamental study to apply a novel double-network (DN) hydrogel to develop the artificial cartilage. This hydrogel is composed of two independently crosslinked hydrophilic networks of poly-2-acrylamido-2-methyl-propanesulfonic acid (PAMPS) and poly-N,N′-Dimetyl acrylamide (PDMAAm) that are physically entangled with each other. This study evaluated the in vivo influence of a PAMPS/PDMAAm DN hydrogel on counterface cartilage in rabbit knee joints and its ex-vivo frictional properties on normal cartilage.
In the first experiment, the DN gel was implanted in a surgically created defect in the femoral trochlea of rabbit knee joints and the left knee was used as the control.
Evaluations using a confocal laser scanning microscopy demonstrated that the DN gel did not affect the surface microstructure (surface roughness, the number of small pits) of the counterface cartilage in vivo at 4 and 12 weeks. The histology also showed the DN gel had no pathological damage on the cartilage matrices and cells at 4 weeks.
However, 2 of the 5 DN gel-implanted knees showed mild irregularity on the counterface cartilage surface at 12 weeks. In the second experiment, the friction property between the normal and artificial cartilage was determined using a joint simulator apparatus. The ex-vivo mean friction coefficient of the DN gel to normal cartilage was 0.029, while that of the normal-to-normal cartilage articulation was 0.188. The coefficient of the DN gel-to-normal cartilage articulation was significantly lower that of the normal-to-normal cartilage articulation (p< 0.0001). This study suggested that the PAMPS/PDMAAm DN gel has very low friction coefficient on normal cartilage and has no significant detrimental effects on counterface cartilage in vivo, and can be a promising material to develop the artificial cartilage.
Correspondence should be addressed to Diane Przepiorski at ISTA, PO Box 6564, Auburn, CA 95604, USA. Phone: +1 916-454-9884; Fax: +1 916-454-9882; E-mail: ista@pacbell.net
