Abstract
Introduction: Embryonic stem cells are pluripotent cells derived from internal mass cell (ICM, Internal Cell Mass) of embryon to the first stages of development (blastocisti. The present study has two goals: 1) to isolate, to cultivate, and to characterize embryonic stem cells derived from blastocisti of sheep produced in vitro. 2) to repair the articular cartilage using stem like cells veicolated on fibrin glue.
Materials and Method: In six sheep in correspondence of medialis femoral condilo, at first, it has been produced an osteochondral full thickness lesion and subsequently it is proceeded to the implant of stem like cells, previously isolated by immunosurgey technique and seeded on the fibrin glue. The new tissue obtained, it has been estimated using the ICRS classification, and undergone to a biomechanical analysis by the Artscan 200 series.
Conclusions: It is possible to obtain stem like cells from sheep embryo’s, produced in vitro with elevated differentiative capacity. The passage in the fibrinogeno and then the added of trombina it doesn’t alter the property of cells, rendering therefore the complex stem cellsfibrin glue, a possible candidate for the repair of cartilage lesions.
Correspondence should be addressed to Ariella Neustadt at Studio EGA, Professional Congress Organisers, Viale Tiziano, 19 - 00196, Rome - Italy.
