Abstract
The identification of different substances able to promote a cellular response in terms of proliferation and differentiation the so-called “morphogenetic proteins”, has expanded research, aiming to identify the cellular elements that produce these proteins, in order to find a source for clinical application.
Platelets have been identified as the main source of morphogenetic protein production: they can be separated in human blood samples and thus it is possible to create a concentration of these elements that, used both in bone as well as in soft tissues, promote a cellular response useful for tissue repair in terms of bone formation and soft tissue regeneration.
Our experience takes into account different fields of application of this new technology: revision surgery, non-union treatment and repair of soft tissue in 18 patients.
The same manufacturing process was utilized for all cases: ”Haemonetics” MCS”+ technology for apheresis, concentration of platelets and plasma/cryoprecipitate obtained, then mixed with autologous thrombin and calcium gluconate to obtain a gel. Usually, for bone surgery, platelet gel is mixed with an autologous iliac crest bone graft or, in some instances, with a homologous bone bank graft, usually morcelized chips.
For soft tissue applications, after surgical débridement, the gel is directly applied over the site and covered by a soft bandage. No adverse effects have been observed: good results, in terms of bone healing and soft tissue repair, were obtained in all cases.
