ROLE OF OPIOID RECEPTORS IN IN-VITRO CHONDROCYTE CELL CULTURES

Abstract

Introduction: Osteoarthritis is the most common joint disease in the world. Biochemical and genetic factors as well as mechanical stress contribute to lesions in the cartilage. The present study analyses the effect of b-Endorphin on the cells of articular cartilage.

Materials and methods: We used rat articular cartilage for the study. After tripsinizing the cartilage and isolating the chondrocytes the cells were cultured in a culture medium. B-Endorphin was dissolved in the culture medium at concentrations of 1 and 10 mM. Only the culture medium was added to the control wells. Naloxone 1 mM was added for co-treatment with b-Endorphin and naloxone. Thirty minutes later, b-Endorphin was added, thus blocking its receptors.

Results: We studied the effect of this procedure on chondrocytes’ proliferating activity and on the proteoglycan synthesis of the extracellular matrix. An increase was observed in the incorporation of 3H-Thymidine, which in turn reflected an increase in the chondrocytes’ proliferating activity. In addition, 35S incorporation analyses were made of cultures which assessed proteoglycan synthesis which showed an increase in the extracellular-matrix forming activity. Differences between the groups with b-endorphin, b-endorphin + naloxone and the control group were found to be highly significant (p< 0.01).

Conclusions: B-endorphin has a stimulating effect upon chondrocytes and proteoglycans present in the extracellular matrix in culture. These stimulating effects are mediated by the interaction with a specific opioid receptor, present in the articular cartilage cells. It may be conceived that trophic stimulation of cartilage cells in the early stages of the disease might partly mitigate the loss of joint surface.