DIRECT MEASUREMENT OF BACTERIAL PENETRATION THROUGH SURGICAL GOWNS: A NEW METHOD

Abstract

The rate of deep infection following primary joint replacement has reduced to below 1%, but the cost remains high. The surgical team is the most important source of bacteria causing infection. All surgical gowns are susceptible to penetration by these organisms, which may then spread to the wound via the surgeon’s hands or contact with wet drapes without ever being airborne.

There is insufficient clinical data on the penetration of bacteria through surgical gowns, in part due to the difficulty of in vivo measurement. A simple new method was developed, using petri dishes filled with horse blood agar that were attached to the outside of the gown material. This was used to assess bacterial penetration through disposable spun-bonded polyester gowns and re-usable woven polyester gowns during normal use.

There was a significant difference between the two gown types when tested in the axilla (p < 0. 05), the groin (p < 0. 05) and the peri-anal region (p < 0. 01), with the disposable gowns performing to a higher standard.

Re-usable gowns demonstrated significant variation in penetrability. This is most likely to be due to the number of laundering and sterilisation cycles that they had undergone. Unless the continued satisfactory performance of multiple-use gowns can be guaranteed, they may be unsuitable for use in orthopaedic implant surgery.