Search | Bone & Joint

Results 1 - 2 of 2

Results per page:

Applied filters

Access

Journals

Abstracts

Dates

Specialties

Bone & Joint Research

Vol. 7, Issue 7 | Pages 457 - 467

1 Jul 2018

A potential key role for alpha-haemolysin of Staphylococcus aureus in mediating chondrocyte death in septic arthritis

Smith IDM Milto KM Doherty CJ Amyes SGB Simpson AHRW Hall AC

Full Access

View article Download PDF

Objectives

Staphylococcus aureus (S. aureus) is the most commonly implicated organism in septic arthritis, a condition that may be highly destructive to articular cartilage. Previous studies investigating laboratory and clinical strains of S. aureus have demonstrated that potent toxins induced significant chondrocyte death, although the precise toxin or toxins that were involved was unknown. In this study, we used isogenic S. aureus mutants to assess the influence of alpha (Hla)-, beta (Hlb)-, and gamma (Hlg)-haemolysins, toxins considered important for the destruction of host tissue, on in situ bovine chondrocyte viability.

Methods

Bovine cartilage explants were cultured with isogenic S. aureus mutants and/or their culture supernatants. Chondrocyte viability was then assessed within defined regions of interest in the axial and coronal plane following live- and dead-cell imaging using the fluorescent probes 5-chloromethylfluorescein diacetate and propidium iodide, respectively, and confocal laser-scanning microscopy.

Bone & Joint Research

Vol. 2, Issue 3 | Pages 58 - 65

1 Mar 2013

Reducing surgical site infection in arthroplasty of the lower limb

Johnson R Jameson SS Sanders RD Sargant NJ Muller SD Meek RMD Reed MR