We attempted to repair full-thickness defects in the articular cartilage of the trochlear groove of the femur in 30 rabbit knee joints using allogenic cultured
The aim of this study was to determine whether subchondral bone influences in situ
Ketamine has been used in combination with a
variety of other agents for intra-articular analgesia, with promising results.
However, although it has been shown to be toxic to various types
of cell, there is no available information on the effects of ketamine
on
Human articular cartilage samples were retrieved from the resected material of patients undergoing total knee replacement. Samples underwent automated controlled freezing at various stages of preparation: as intact articular cartilage discs, as minced articular cartilage, and as
The gelatin-based haemostyptic compound Spongostan was tested as a three-dimensional (3D)
Sex hormones play important roles in the regulation of the proliferation, maturation and death of
The aim of this study was to determine whether exposure of human articular cartilage to hyperosmotic saline (0.9%, 600 mOsm) reduces in situ
Post-traumatic arthritis is a frequent consequence of articular fracture. The mechanisms leading to its development after such injuries have not been clearly delineated. A potential contributing factor is decreased viability of the articular chondrocytes. The object of this study was to characterise the regional variation in the viability of
Desiccation of articular cartilage during surgery is often unavoidable and may result in the death of
We attempted to characterise the biological quality
and regenerative potential of
Gene therapy with insulin-like growth factor-1 (IGF-1) increases matrix production and enhances
Bone morphogenetic protein (BMP) has a crucial role in osteochondrogenesis of bone formation as well as in the repair of fractures. The interaction between hedgehog protein and BMPs is inferred from recent molecular studies. Hedgehog genes encode secreted proteins which mediate patterning and growth during skeletal development. We have shown that Indian hedgehog gene (Ihh) is expressed in cartilage anlage and later in mature and hypertrophic
Chondrocytes at the lower zone of the growth plate must be eliminated to facilitate longitudinal growth; this is generally assumed to involve apoptosis. We attempted to provide definitive electron-microscopic evidence of apoptosis in
1. Twelve trephine specimens of articular cartilage and subchondral bone taken from six fresh osteoarthritic femoral heads were incubated in a medium containing tritiated thymidine, and autoradiographs were prepared from serial sections five microns thick. 2. Scattered labelling of
In an attempt to repair articular cartilage, allograft articular chondrocytes embedded in collagen gel, were transplanted into full-thickness defects in rabbit articular cartilage. Twenty-four weeks after the transplantation, the defects were filled with hyaline cartilage, specifically synthesising Type II collagen. These
This in vivo controlled laboratory
study was performed to evaluate various intra-articular clinical
injection regimes that might be less toxic than some in
vitro studies suggest. We hypothesised that low-concentration,
preservative-free, pH-balanced agents would be less toxic than high-concentration
non-pH-balanced agents with preservatives, and that injections of
individual agents are less toxic than combined injections. The left
knees of 12- to 13-week-old Sprague–Dawley rats were injected once
with eight different single agents, including low and high concentrations of
ropivacaine and triamcinolone, alone and in combination, as well
as negative and positive controls. The rats were killed at one week
or five months, and live–dead staining was performed to quantify
the death of
We have developed a novel, two-layered, collagen matrix seeded with
Matrix metalloproteinases (MMPs), responsible
for extracellular matrix remodelling and angiogenesis, might play
a major role in the response of the growth plate to detrimental
loads that lead to overuse injuries in young athletes. In order
to test this hypothesis, human growth plate
The management of failed autologous chondrocyte
implantation (ACI) and matrix-assisted autologous
Matrix-induced autologous