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Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XXXVII | Pages 553 - 553
1 Sep 2012
Lustig S Allais E Boisset S Ferry T Tigaud S Neyret P Laurent F
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Introduction

Microbiological diagnosis of bone and joint infections (BJIs) currently relies on standard cultures which are time consuming and lack sensitivity. Various molecular approaches have been described and allowed improvement of BJI diagnosis. This study evaluated for the first time the performance of a DNA microarray-based assay (Prove-itâ„¢ Sepsis assay, PISA) for the rapid (<6 hours) detection and identification of 50 different species involved in BJI directly from clinical samples.

Material and methods

We retrospectively selected 130 bone and joint samples (67 synovial fluids and 63 bone biopsies) including 114 positive and 16 negative samples. The microbiological diagnosis had been previously established either by culture(C+, n=53) or by PCR16S and sequencing when culture was negative (C-/PCR+). The positive samples were selected to match the species targeted on the DNA microarray. DNA extraction was performed before proceeding to PISA amplification and hybridization on every selected sample.


Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XXXVII | Pages 552 - 552
1 Sep 2012
Lustig S Laurent F Bouaziz A Blanc-Pattin V Rasigade J Ferry T Tigaud S Neyret P
Full Access

Introduction

Rapid identification of bacteria from extemporaneous samples would greatly help management of prosthesis joint infection. The aim of the present retrospective study was to evaluate a new molecular assay (GeneXpert MRSA-SA SSTI (Cepheid)) for detecting Staphylococcus aureus (SA) and methicillin resistance directly from bone and joint samples in less an hour (58 minutes).

Material et method

Retrospective study using 91 frozen samples (76 patients) of joints (n=24), bone biopsies (n=42) and tissue biopsies (n=25):

SA positive samples: n=72 (methicillin susceptible SA (MSSA), n=63; methicillin resistant MRSA, n=9)

SA positive samples: n=19

The results were compared with routine results (culture in solid and liquid medium, identification and susceptibility test) from each participating lab.