When using radiation intraoperatively, a surgeon should aim to maintain the dose as low as reasonably achievable to obtain the diagnostic or therapeutic goal. The UK Health Protection Agency reported mean radiation dose-area-product (DAP) of 4 Gy cm2 for hip procedures. We aimed to investigate factors associated with increased radiation exposure in fixation of proximal femur fractures. We assessed 369 neck of femur fractures between April 2019 and April 2020 in one district general hospital. Fractures were classified as extracapsular or intracapsular and into subtypes as per AO classification. Data was collected on type of fractures, implants used, level of surgeon, duration of surgery and DAP. Types of fractures were subclassified as complex (multifragmentary, subtrochanteric and reverse oblique) or simple.Introduction and Objective
Materials and Methods
Human mesenchymal stem cells are considered the golden standard for clinical application in regenerative medicine for their multilineage differentiation potential, best candidates to treat diseases such as osteoarthritis and osteogenesis imperfecta. In the past few years several molecules have been described to induce the hMSCs differentiation into osteo cell progenitors, mainly discovered by screening of single metabolites bioactivity. However, hMSCs osteogenic differentiation potential is still poor, and the discovery of differentiation inducers with high efficiency is needed. Thanks to automated processes, High Throughput Screening (HTS) strategies shorten the metabolites bioactivity investigation timeline, allowing testing of many molecules simultaneously. In this work, reliable assays for natural products bioactivity investigation detection were developed using HTS methodologies and validated by testing 15 purified compounds derived by marine fungi and sponges. The HTS cytotoxicity investigation using HepG2 cells allowed to test in a single experiment, 15 metabolites in 4 concentrations ranging from 1 to 20µM. Low cytotoxicity was detected for metabolites concentrations from 1 to 10µM and so set as treatment concentrations to be tested in further assays. Anti-inflammatory bioactivity was tested on THP1 cells triggered by LPS. Five out of 15 metabolites showed to prevent the LPS induced THP1 inflammatory activation by lowering the TNF-α production. The metabolites pro-osteogenic potential was investigated using hMSCs: their differentiation was evaluated by calcium mineralization after 10 days differentiation. Pro-osteogenic molecules were not detected in this screening, but the method validation represents a powerful tool for future natural product and synthetic molecules libraries screenings.
Claw toes are treated by a variety of soft tissue and bony proceduresbased on the severity of the deformity. We evaluated the results of Stainsby procedure for claw toes. This is a retrospective analysis of the results of Stainsby procedure for claw toes of the foot done by a single surgeon over a 10 year period. All patients who had claw toes,secondary to Rheumatoid and Non rheumatoid causes and treated by this procedure were included in the study. All the patients operated between Jan 1995 -Dec 2004 and who had minimum follow-up of 6 months after surgery were included in the study. Follow up evaluation was by clinical examination, review of case notes and telephone conversation. 42 patients underwent this procedure of which 38 were available for evaluation. Average follow-up was 43.5 months (6–110months). 26 rheumatoid and 21 non-rheumatoid feet were evaluated based on the AOFAS score. The mean AOFAS score was 76.5. The scores for the Rheumatoid and Non-Rheumatoid groups were 81.5 and 72.6 respectively.81% were satisfied with the result of the operation and 83% would recommend this surgery for friends and relatives. Six patients had superficial infection, 2 had broken k-wires, 2 had DVT, and 2 had recurrence of deformity. We conclude that Stainsby procedure for claw toes is a procedure which has good results in the long term and can be taken up as a procedure of choice for severe claw toes.
Claw toes are treated by a variety of soft tissue and bony proceduresbased on the severity of the deformity. We evaluated the results of Stainsby procedure for claw toes. This is a retrospective analysis of the results of Stainsby procedure for claw toes of the foot done by a single surgeon over a 10 year period. All patients who had claw toes, secondary to Rheumatoid and Non rheumatoid causes and treated by this procedure were included in the study. All the patients operated between Jan 1995 -Dec 2004 and who had minimum follow-up of 6 months after surgery were included in the study. Follow up evaluation was by clinical examination, review of case notes and telephone conversation. 42 patients underwent this procedure of which 38 were available for evaluation. Average follow-up was 43.5 months (6–110months). 26 rheumatoid and 21 non-rheumatoid feet were evaluated based on the AOFAS score. The mean AOFAS score was 76.5. The scores for the Rheumatoid and Non-Rheumatoid groups were 81.5 and 72.6 respectively.81% were satisfied with the result of the operation and 83% would recommend this surgery for friends and relatives. Six patients had superficial infection, 2 had broken k-wires, 2 had DVT, and 2 had recurrence of deformity. We conclude that Stainsby procedure for claw toes is a procedure which has good results in the long term and can be taken up as a procedure of choice for severe claw toes.
To evaluate patient satisfaction and expectations of surgery following forefoot arthroplasty.
Between October 1993 and June 1999, forefoot arthroplasty (Kate/Kessel/Kay procedure) was performed or directly supervised by the senior author (D. McBride) in a cohort of 55 patients. All had inflammatory arthritis and had failed non-operative management. The clinical result was assessed using a self-administered patient satisfaction questionnaire. The questionnaire asked patients to rate their level of satisfaction in terms of pain relief, wound healing, stiffness and appearance. The patients expectations from the surgery in terms of their level of disability and the achievement of the operation in addition to their pain experience following their operation was assessed.
Median time to follow-up was 41 months (range seven to seventy-seven). Forty-three of the 55 patients returned the completed questionnaire. The median age at operation of the respondents was 59 years (range 42 to 69) compared with 49 years (range 44 to 63) for the non-respondents. Of the 43 respondents, 30 were female and 13 male. In terms of their expectations of the surgery, 20 (47%) stated that the operation had achieved what they had expected while 10 (23%) considered the operation to have achieved more than they had expected. The level of disability following their operation was as expected in 21 (49%), more than expected in 11 (26%) and less than expected in four (9%). 23 (55%) noted no change to their walking capacity while it had increased in 11 (26%) and decreased in eight (19%). There were two wound haematomas, five superficial wound infections and three cases of delayed wound healing which extended the post-operative hospital stay. Seventy-nine percent of patients however reported complete satisfaction with their wound healing. In those patients that had reported having had pain (n=31) at some time following their operation, two (7%) had experienced it for less than seven days, four (13%) for between one and four weeks, one (3%) for between one and three months and 23 (77%) for more than three months.
Overall patients were generally satisfied with their operation in terms of pain relief, wound healing and appearance. Additionally, in the majority of patients, the achievements of the procedure and the associated disability were as expected. Previous authors have outlined the various surgical factors, which are said to lead to a good outcome. Whilst it is important to bear these factors in mind we have found that some of our patients appeared to have a good result when these criteria were not met. While other patients meeting these criteria were not necessarily satisfied. This suggests areas for further research.
