Purpose: To define the positions of the attachments of the anteromedial (AM) and posterolateral (PL) bundles of the ACL facilitating accurate tunnel placement during two-bundle reconstruction.

Methods: The positions of the femoral and tibial attachments of the AM and PL bundles was determined in 7 fresh-frozen, unpaired, cadaveric knees by 6 independent observers, using landmarks visible at arthroscopy. This included, on the tibia, the retro-eminence ridge (lying just anterior to the PCL), a bony landmark that could be reliably identified arthroscopically. Tantallum beads were then inserted so that the bundle attachments could be clearly identified on a plain lateral radiograph of the knee. The position of the centres of the AM and PL attachments were described relative to Amis and Jakob’s line on the tibia and Bernard’s grid on the femur.

Results: The AM femoral attachment lay high and deep in the notch with the most posterior fibres 1.8 mm anterior to the “over–the-top” position. The PL femoral attachment was low and shallow in the notch with the most anterior fibres 2.8 mm from the border of the articular cartilage. The centres of the bundles were 8.2 mm apart. The position of the bundles relative to Bernhard’s grid is shown in figure 1.

On the tibia, the centre of the AM attachment was located 18 mm anterior to the Retro-eminence ridge (RER). The centre of the PL bundle lay 8.4 mm posterior to the centre of the AM bundle. These positions were at 35% and 52% along Amis and Jacob’s line

Conclusions: This study details the morphology of the AM and PL bundle attachments and demonstrates reliable arthroscopic techniques to assist with accurate tunnel placement in reconstruction surgery. In addition, it provides reference data for radiographic evaluation of tunnel placement.