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Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_23 | Pages 76 - 76
1 Dec 2016
UTILITY OF GENE EXPRESSION PATTERN OF TOLL-LIKE RECEPTORS AND IL-1/IL1R FAMILY FOR ASSESSMENT OF PERIPROSTHETIC JOINT INFECTION IN TOTAL JOINT ARTHROPLASTY
Fillerova R Petrackova A Gajdos P Kudelka M Kriegova E Gallo J
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Aim

The diagnosis of periprosthetic joint infection (PJI) in total joint arthroplasty (TJA) remains a serious clinical challenge. Nowadays, limited biomarkers associated with PJI are available. We investigated therefore the utility of gene expression pattern of Toll-like receptors (TLR) and members of interleukin (IL)1/IL1R family, molecules critically involved in the innate immune response to invading pathogens, for detecting PJI in periprosthetic tissues around TJA.

Method

Periprosthetic tissues were collected from 37 patients presenting with PJI and 39 patients having an aseptic failure of TJA. mRNA expression of known TLR receptors (TLR1–10) and 21 members of IL-1/IL-1R family was investigated using an innovative Smartchip Real-Time RT-PCR System*; the data were normalized relative to the housekeeping gene GAPDH. Statistical tests were performed using GraphPad Prism** and bio-data mining methods.