Over the last two decades, anatomic anterior cruciate ligament (ACL) reconstructions have gained popularity, while the use of extraarticular reconstructions has decreased. However, the biomechanical rationale behind the lateral extraarticular sling has not been adequately studied. By understanding its effect on knee stability, it may be possible to identify specific situations in which lateral extraarticular tenodesis may be advantageous. The primary objective of this study was to quantify the ability of a lateral extraarticular sling to restore native kinematics to the ACL deficient knee, with and without combined intraarticular anatomic ACL reconstruction. Additionally, we aimed to characterise the isometry of four possible femoral tunnel positions for the lateral extraarticular sling. Eight fresh frozen hip-to-toe cadavers were used in this study. Navigated Lachman and mechanised pivot shift examinations were performed on ACL itact and deficient knees. Three reconstruction strategies were evaluated: Single bundle anatomic intraarticular ACL reconstruction, Lateral extraarticular sling, Combined intraarticular ACL reconstruction and lateral extraarticular sling. After all stability tests were completed, we quantified the isometry of four possible femoral tunnel positions for the lateral extraarticular sling using the Surgetics navigation system. A single tibial tunnel position was identified and digitised over Gerdy's tubercle. Four possible graft positions were identified on the lateral femoral condyle: the top of the lateral collateral ligament (LCL); the top of the septum; the ideal tunnel position, as defined by the navigation system's own algorithm; and the actual tunnel position used during testing, described in the literature as the intersection of the linear projections of the LCL and the septum over the lateral femoral condyle. For each of the four tunnel positions, the knee was cycled from 0 to 90® of flexion and fiber length was recorded at 30® intervals, therefore quantiying the magnitude of anisometry for each tunnel position. Stability testing: Sectioning of the ACL resulted in an increase in Lachman (15mm, p = 0.01) and mechanised pivot shift examination (6.75mm, p = 0.04) in all specimens compared with the intact knee. Anatomic intraarticular ACL reconstruction restored the Lachman (6.7mm, p = 3.76) and pivot shift (−3.5mm, p = 0.85) to the intact state. With lateral extraarticular sling alone, there was a trend towards increased anterior translation with the Lachman test (9.2mm, p = 0.50). This reconstruction restored the pivot shift to the intact state. (1.25mm, p = 0.73). Combined intraarticular and extraarticular reconstruction restored the Lachman (6.2mm, p = 2.11) and pivot shift (−3.75mm, p = 0.41) to the intact state. There was no significant difference between intraarticular alone and combined intraarticular and extraarticular reconstruction. (p = 1.88) Isometry: The ideal tunnel position calculated by the navigation system was identified over the lateral femoral condyle, beneath the mid-portion of the LCL. The anisometry for the ideal tunnel position was significantly lower (5.9mm; SD = 1.8mm; P<0.05) than the anisometry of the actual graft position (14.9mm; SD = 4mm), the top of the LCL (13.9mm; SD = 4.3mm) and the top of the septum (12mm; SD = 2.4mm). In the isolated acute ACL deficient knee, the addition of a lateral extraarticular sling to anatomic intraarticular ACL reconstruction provides little biomechanical advantage and is not routinely recommended. Isolated lateral extraarticular sling does control the pivot shift, and may be an option in the revision setting or in the lower demand patient with functional instability. Additionally, the location of the femoral tunnel traditionally used results in a significantly more anisometric graft than the navigation's system mathematical ideal location. However, the location of this ideal tunnel placement lies beneath mid-portion of the fibers of the LCL, which would not be clinically feasible.