Introduction and Objectives: The success rate of bone allografts in the medium term when used in cancer surgery is 63% to 90% according to the different series. Our aim was to analyze the results and the complication rate seen in bone allografts as used in our center.

Materials and Methods: We collected follow-up data from 35 patients who received 37 allografts. The variables analyzed include diagnosis, age, bone and side affected, type of allograft, complications, additional surgery, time of follow-up, and allograft and patient survival.

Results: Mean age at surgery: 10.6 years. 48.64% were osteosarcomas, 48.64% were Ewing sarcomas, 2.7% were other diagnoses. 52.94% were strut grafts, 29.41% were osteoarticular, 2.94% were composite, 2.94% were arthrodesis and 11.76% were other types. Of these, 88.88% suffered some type of complication and 81. 48% required additional surgery. We achieved allograft survival in 85.29% of cases with a mean follow-up of 55.45 months. Most frequent complications were non-union (25%), postoperative metastasis (25%) length discrepancy (25%), followed by degenerative arthritis (24%) graft resorption and infection (16.6%)

Discussion and Conclusions: Allografts are a reconstructive option with good results in the medium term. In spite of the high complication rate, additional surgeries applying rescue procedures have made it possible to obtain allograft survival similar to that seen in published series.

Our aim was to evaluate the expression of transcription factors CCAAT/enhancer-binding protein-beta (C/EBP_β) and C/EBP-homologous protein (CHOP) in the growth plate. Proximal tibial epiphyseal growth plates from ten 15-day-old Wistar rats were used. Additionally, anti-proliferating cell nuclear antigen (PCNA), anti-5-bromo-2’-deoxyuridine (BrdU) immunostaining, terminal transferase dUTP nick end-labelling (TUNEL) and nucleolar organiser region-associated proteins (AgNOR) techniques were peformed. The histological morphology of the growth plate from C/EBP_βknock-out mice was also analysed.

The normal growth plate showed that C/EBP_β and CHOP factors are expressed both in the germinative/ upper proliferative and in the lower proliferative zones. Furthermore, BdrU+ and PCNA+ cells were present exclusively in the germinative and proliferative zones, while TUNEL+ and AgNOR+ cells were seen in all three zones of the growth plate. Acellular areas, hypocellularity, the increase in cell death and anomalies in the architecture of the cell columns were observed in the growth plates of C/EBP_β (−/ −) knockout mice.

We suggest that C/EBP_β and CHOP transcription factors may be key modulators participating in the chondrocyte differentiation process in the growth plate.