Advertisement for orthosearch.org.uk
Results 1 - 8 of 8
Results per page:
Applied filters
Content I can access

Include Proceedings
Dates
Year From

Year To
Orthopaedic Proceedings
Vol. 99-B, Issue SUPP_1 | Pages 124 - 124
1 Jan 2017
Sakane M Tsukanishi T Funayama T Onishi S Ozeki E Hara I Yamazaki M
Full Access

Photodynamic therapy (PDT) uses the strong cytotoxicity of singlet oxygen and hyperthermia produced by irradiating excitation light on a photosensitizer. The phototoxic effects of indocyanine green (ICG) and near-infrared light (NIR) have been studied in different types of cancer cells. Plasma proteins bind strongly to ICG, followed by rapid clearance by the liver, resulting in no tumor-selective accumulation after systemic administration. Kimura et al. have proposed using a novel nanoparticle labeled with ICG (ICG-lactosome) that has tumor selective accumulation owing to enhanced permeability and retention (EPR) effect. In this study, we investigated the efficacy of PDT using ICG-lactosome and NIR for a bone metastatic mouse model of breast cancer.

Cells from the human breast cancer cell line, MDA-MB-231 were injected into the right tibia of 26 anesthetized BALB/C nu/nu mice at a concentration. The mice were then randomly divided into three groups: the PDT group (n = 9), the laser (laser irradiation only) group (n = 9), and the control group (n = 8). PDT was performed thrice (7, 21, 35 days after cell inoculation) following ICG-lactosome administration via the tail vein 24 hours before irradiation. The mice were percutaneously irradiated with an 810-nm medical diode laser for 10 min. In the laser group, mice were irradiated following saline administration 24 hours before irradiation. Radiographic analysis was performed for 49 days after cell inoculation. The area of osteolytic lesion was quantified. The right hind legs of 3 mice were amputated 24 hours after the third treatment. Histological analysis was performed using hematoxylin-eosin staining and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining of sagittal sections. The data was analyzed using Tukey-Kramer post-hoc test. P-value of <0.05 was considered significant.

X-ray on day 49 of the three groups are considered. The area of osteolytic lesion in the PDT group (7.9 ± 1.2 mm2: mean ± SD) was significantly smaller than that of the control (11.4 ± 1.4 mm2) and laser (11.9 ± 1.2 mm2) groups. In histological findings, we observed many TUNEL-positive cells in the metastatic tissue 24 hours after PDT. In the control and laser groups, TUNEL-positive cells were occasionally observed.

We have previously reported the effect of ICG-lactosome-enhanced PDT on the cytotoxicity of human breast cancer cells in vitroand on the delay of paralysis in a rat spinal metastasis model. In this study, we demonstrated the inhibitory effect of ICG-lactosome-enhanced PDT on bone destruction caused by human breast cancer cells in vivo. This PDT induced apoptosis and necrosis in the tumor cells. Intralesional resection is often performed for spinal metastases in an emergency. The residual tumor may regrow and cause neurological deficits. We believe that ICG-lactosome-enhanced PDT can decrease the rate of local recurrence through reduction of the residual tumor. PDT with ICG-lactosome and NIR had an inhibitory effect on the growth of bone metastasis of a human breast cancer.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_10 | Pages 110 - 110
1 May 2016
Wada H Mishima H Yoshizawa T Sugaya H Nishino T Yamazaki M
Full Access

Introduction

Hydroxyapatite and poly-L-lactide (HA/PLLA) composites are osteoconductive and biodegradable. They have already been used clinically to treat fractured bones by inducing osteosynthesis and serving as the bone filling material. During revision of total hip arthroplasty, we have grafted bone onto the bone defect and covered it with an HA/PLLA mesh instead of using a metal mesh on the non-load bearing portion of the cup (Figure 1). However, whether the interface between the HA/PLLA and the titanium alloy cup was stable remains unclear.

Objectives

The purpose of this study was to determine and compare the histological osteoconductivity and osteoinductivity of HA/PLLA and titanium alloy.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_10 | Pages 79 - 79
1 May 2016
Totsuka S Mishima H Wada H Yoshizawa T Sugaya H Nishino T Yamazaki M
Full Access

Introduction

Migration of the trial femoral head is a rarely occurring complication of total hip arthroplasty (THA) performed using the anterolateral approach (ALA). This migration of the trial femoral head under the rectus femoris is extremely risky because of the anatomical situation. Analyzing the morphological character of a case of migration may help us to avoid this risk.

Objective

We analyzed the three-dimensional bone morphology using computed tomography (CT) scan images to investigate the physiological characteristics of five migration cases.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_4 | Pages 101 - 101
1 Jan 2016
Wada H Mishima H Sugaya H Nishino T Yamazaki M Hyodo K
Full Access

Introduction

The initial stress distribution in the femur after total hip arthroplasty (THA) influences the remodeling of the bone and the clinical results. We conducted thermoelastic stress analysis to evaluate the surface stress distribution in femurs after THA in vitro and elucidated the changes in the stress distribution that were due to the stem design. Using this method, we can analyze the change in the sum of the principal stresses on the basis of the changes in temperature at the bone surface and visualize the change three-dimensionally.

Objectives

We compared the thermoelastic stress analysis results with the clinical results of specially shaped stems that have two flanges, one anterior and one posterior.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_4 | Pages 9 - 9
1 Jan 2016
Shimizu Y Kamada H Sakane M Aikawa S Tanaka K Mishima H Kanamori A Eguchi K Mutsuzaki H Wadano Y Ochiai N Yamazaki M
Full Access

Background

Venous thromboembolisms are serious complications of arthroplasty of the lower extremities. Although early ambulation and active leg exercise is recommended, postoperative patients with surgical pain have difficulty in moving their legs. Therefore, we developed a novel leg exercise apparatus (LEX) to facilitate active leg movement even during the early postoperative period (Fig 1). LEX is a portable apparatus that allows patients to actively move their legs while in the supine position. LEX enables dorsiflexion, plantar flexion, combined eversion and inversion of the ankle, and multi-joint movement of the leg.

Objectives

To describe how LEX facilitates active movement of the leg and thereby increases venous flow in the lower extremities.


Orthopaedic Proceedings
Vol. 96-B, Issue SUPP_11 | Pages 349 - 349
1 Jul 2014
Hyodo K Yoshioka T Akaogi H Sugaya H Aoto K Wada H Sakai S Yamazaki M Mishima H
Full Access

Introduction

The goal of joint-preserving surgery for the treatment of osteonecrosis of the femoral head (ONFH) is to delay or prevent osteoarthritic development. Bone marrow is a source of osteogenic progenitors that are key elements in the process of bone formation and fracture healing. We established an easy-to-use method using a conventional manual blood bag centrifugation technique traditionally used for extracting buffy coats, for concentration of nucleated cells and platelets from clinical bone marrow aspirates to obtain osteogenic progenitors and growth factors. However, it is unclear whether the surgical goals are really achieved and if so in which patients. The purpose of this study was to identify demographic, clinical, and radiographic factors predicting total hip arthroplasty (THA) conversion after CABMAT for the treatment of idiopathic ONFH.

Methods

We retrospectively reviewed 123 patients (213 hips) who had CABMAT between 2003 and 2010. Sixty-five subjects (115 hips) were male and 58 (98 hips) were female with an average age at the time of CABMAT of 40.1 years. Of the 213 hips, 143 hips in 78 patients had corticosteroid-induced ONFH, 46 hips in 27 patients had alcohol-associated, and 24 hips in 18 patients had no etiological factors could be detected. The mean follow-up period was 60.5 months. The endpoint of evaluation was set as the time point which the patient required additional surgery (THA) depending on the spontaneous hip pain, x-ray change, and social back ground. The following factors were investigated: age, sex, body mass index (BMI), unilateral or bilateral, etiological factors, preoperative classification and staging, visual analogue scale (VAS), JOA clinical score. The 213 hips were divided into two groups: a THA conversion (THA) group and a non-THA conversion (non-THA) group. A multivariate analysis was performed using a logistic regression model.


Orthopaedic Proceedings
Vol. 96-B, Issue SUPP_11 | Pages 53 - 53
1 Jul 2014
Wada H Mishima H Hyodo K Yamazaki M
Full Access

Summary Statement

We used three-dimensional software to assess different anatomic variables in the femur. The canal of Femur twisted slightly below the lesser trochanter in cases with a larger angle of anteversion.

Introduction

Accurate positioning of the joint prosthesis is essential for successful total hip arthroplasty (THA). To aid in tailoring of the prosthesis, we used three-dimensional software to assess different anatomic variables in the femur.


Orthopaedic Proceedings
Vol. 96-B, Issue SUPP_11 | Pages 111 - 111
1 Jul 2014
Tsukanishi T Funayama T Yamazaki M Onishi S Ozeki E Hara I Sakane M
Full Access

Summary

Photodynamic therapy with ICG lactosome and near-infrared light has phototoxic effects on human breast cancer cells. With the same total energy, phototoxic effects depend on output of irradiation light rather than irradiation time.

Introduction

The phototoxic effects of indocyanine green (ICG) and near-infrared light have been studied in various fields. Plasma proteins bind strongly to ICG, which is followed by rapid clearance by the liver, resulting in no tumor selectivity after systemic administration. We have proposed a novel nanocarrier labeled with ICG (ICG lactosome) that has tumor selectivity due to its enhanced permeation and retention (EPR) effect. The aim of this study was to investigate in vitro phototoxic effects and to optimise the irradiation conditions by changing the output and time of near-infrared light as excitation light.