Introduction: The amount of loading on the cruciate ligaments depends on the tension of the external muscular structures. In vivo studies using EMG have observed a proprioreceptive eccentric co-contraction of the hamstrings during isokinetic knee extension motion. This antagonistic co-contraction increases the quadriceps force necessary to produce the same extension moment on the knee, whereas the loading on the anterior cruciate ligament was measured to be reduced, with the loading on the posterior cruciate ligament to be increased. The objective of this study was thus to investigate the effect of simulated proprioreceptive co-contraction of the hamstrings muscles on quadriceps force, as well as on the relative loading on the cruciate ligament structures during knee extension under dynamic conditions and physiologic loads.
Methods: Five fresh frozen knee specimen were tested in isokinetic extension. Bow shaped loading transducers were fixed in the medial fibres of the anterior (ACL) and posterior cruciate ligament (PCL). The test cycle simulated an isokinetic extension cycle from 120 degrees of flexion to full extension, a hydraulic cylinder thereby applied sufficient force to the quadriceps tendon in a closed-loop control cycle to produce a constant extension moment of 31 Nm about the knee. A second hydraulic cylinder simulated a 200 N co-contraction force of the hamstrings tendons. The loading on the ACL and PCL was first measured in the absence of hamstrings force, and subsequently under constant co-contractive flexion force.
Results: In the absence of hamstring tension, the maximum quadriceps force was 1190 N ( SD 204 N) at 105 degrees of knee flexion. The loading on the ACL was reduced at larger flexion angles, the loading pattern of the PCL showed an inverse relationship with less loading at full extension. The maximum loading in the ACL was 161 N (SD138 N) and maximum tension in the PCL was 38.2 N (SD 34.9). With hamstring co-contraction, maximum quadriceps force increased 19.9 % ( SD 21.0% p= 0.33), maximum tension in the ACL decreased 71.9% (SD 74.3%, p=0.03), and maximum tension in the PCL increased 73.0% (SD 40.9%, p=0.03).
Discussion: This experimental setup enabled direct in vitro measurement of ACL and PCL loading during simulated isokinetic extension motions. The loading on the ACL was dependent on the knee flexion angle. We observed that co-contraction of the hamstrings reduces loading on the anterior cruciate ligament without a significant concomitant increasing the quadriceps muscle force. Our results support the hypothesis that antagonistic co-contraction of the hamstrings during extension of the knee provides an important protective function. In contrast, loading in the posterior cruciate ligament increased during hamstring activation at higher knee flexion angles.
