Postero-lateral rotator instability (PLRI) is the most common pattern of recurrent elbow instability. Unfortunately, current imaging to aid PLRI diagnosis is limited. We have developed an ultrasound (US) technique to measure ulnohumeral joint gap with and without stress of the lateral ulnocollateral ligament. We sought to define lateral ulnohumeral joint gap measurements in the resting and stressed state to provide insight into how US may aid diagnosis of PLRI. Sixteen elbows were evaluated in eight healthy volunteers. Lateral ulnohumeral gap was measured on US in the resting position and with posterolateral drawer stress test maneuver applied. Joint laxity was calculated as the difference between stress and rest conditions. Measurements were performed by two independent readers with comparison performed between stress and rest positions. A highly significant difference in ulnohumeral gap was seen between stress and rest conditions (Reader 1: p < 0 .0001 and Reader 2: p=0.0002) with median values of 2.93 mm and 2.50 mm at rest and 3.92 mm and 3.40 mm at stress for Reader 1 and 2 respectively. Median joint laxity was 1.02 mm and 0.74 mm respectively for each reader. Correlation and agreement between readers was good. This study provides key new insight into use of US for diagnosis as PLRI as it defines normal ulnohumeral distances and demonstrates widening when applying the posterolateral drawer stress maneuver. Further evaluation of this technique is required in patients with PLRI.