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Purpose: To describe the percutaneous transpedicular biopsy technique as a novel way of approaching lesion of the thoracic and lumbar spine, to determine the amount of bone retrievable through the pedicle and its diagnostic yield. Material and Methods: Seventy-nine patients underwent 84 biopsies. Seventy-seven procedures were performed with ßuoroscopic guidance arid seven with CT guidance. Seventy-one biopsies underwent under local anesthesia and ten under general anesthesia. Age range of patient was from 3 to 81 years. Results: Adequate specimens for correct diagnosis were obtained in 80 of the 84 patients with the following diagnoses. Pyogenic spondylodiscitis 31, tuberculosis 4, coccidiomycosis 2, echinococcus cyst 1, blastomycosis 1, brucella 4, primary neoplasm 7, metastatic neoplasms 16, osteoporotic fractures 8, osseous repair for insufþciency fractures 5, Pagetñs disease 1. The 4 negative biopsies subsequently proven to be Ç false negative È and were related to faulty biopsy techniques. Conclusion: Pitfalls can be avoided when adhering to the details of our technique. These pitfalls can occur while retrieving the instrumentation without simultaneous withdrawal of the guiding pin; crushing pathological soft tissue against sclerotic or normal bone; or when encountering a sclerotic lesion distal to normal bone without using a sequential type of biopsy specimen-retrieval technique. Any type of bleeding is controllable. The approach is a safe, efþcacious and cost effective and avoids so the problems such low diagnostic yield nerve root injury, pneumothorax and hematoma encountered with conventional needle technique