Continuous local antibiotic perfusion (CLAP) has recently attracted attention as a new drug delivery system for orthopaedic infections. CLAP is a direct continuous infusion of high-concentration gentamicin (1,200 μg/ml) into the bone marrow. As it is a new system, its influence on the bone marrow is unknown. This study aimed to examine the effects of high-concentration antibiotics on human bone tissue-derived cells. Cells were isolated from the bone tissue grafts collected from six patients using the Reamer-Irrigator-Aspirator system, and exposed to different gentamicin concentrations. Live cells rate, apoptosis rate, alkaline phosphatase (ALP) activity, expression of osteoblast-related genes, mineralization potential, and restoration of cell viability and ALP activity were examined by in vitro studies.Aims
Methods
This study aimed to evaluate the accuracy of implant placement with robotic-arm assisted total hip arthroplasty (THA) in patients with developmental dysplasia of the hip (DDH). The study analyzed a consecutive series of 69 patients who underwent robotic-arm assisted THA between September 2018 and December 2019. Of these, 30 patients had DDH and were classified according to the Crowe type. Acetabular component alignment and 3D positions were measured using pre- and postoperative CT data. The absolute differences of cup alignment and 3D position were compared between DDH and non-DDH patients. Moreover, these differences were analyzed in relation to the severity of DDH. The discrepancy of leg length and combined offset compared with contralateral hip were measured.Aims
Methods
With recent progress in cancer treatment, the number of advanced-age patients with spinal metastases has been increasing. It is important to clarify the influence of advanced age on outcomes following surgery for spinal metastases, especially with a focus on subjective health state values. We prospectively analyzed 101 patients with spinal metastases who underwent palliative surgery from 2013 to 2016. These patients were divided into two groups based on age (< 70 years and ≥ 70 years). The Eastern Cooperative Oncology Group (ECOG) performance status (PS), Barthel index (BI), and EuroQol-5 dimension (EQ-5D) score were assessed at study enrolment and at one, three, and six months after surgery. The survival times and complications were also collected.Aims
Methods
Several reports demonstrated the overcoverage of the anterior acetabulum. Anterior CE angle over 46°may be a probable risk factor for pincer FAI syndrome after a rotational acetabular osteotomy. In addition, a highly anteverted femoral neck, reported as a risk factor for posterior impingement, has been found in DDH patients. These findings indicate proper acetabular reorientation is essential to avoid anterior or posterior impingement after periacetabular osteotomy (PAO). The aim of this study was to evaluate the relationship between acetabular three-dimensional (3D) alignment reorientation and clinical range of motion (ROM) after periacetabular osteotomy (PAO). A total of 53 patients who underwent curved PAO (CPO) for DDH from January 2014 to April 2017 were selected. Three (5.7%) of them were lost to follow-up. Therefore, the data from 58 hips, contributed by 50 patients (44 women and 6 men), were included in the analysis. Pre- and postoperative computed tomography (CT) scans from the pelvis to the knee joint were performed and transferred to a 3D template software (Zed Hip; Lexi, Tokyo, Japan). The pelvic plane axis was defined according to the functional pelvic plane. The pre- and postoperative lateral and anterior 3D center-edge (CE) angles were measured on the coronal and sagittal views through the center of the femoral head. The pre- and postoperative 3D center-edge (CE) angles and femoral anteversion were measured and compared with clinical outcomes, including postoperative ROM.Introduction
Methods
The influences of posterior tibial slope on the knee kinematics have been reported in both TKA and UKA. We hypothesized the posterior tibial slope (PTS) would affect the sagittal knee alignment after UKA. The influences of PTS on postoperative knee extension angle were investigated with routine lateral radiographies of the knee after UKA. Twenty-four patients (26 knees; 19 females, 7 males) underwent medial UKA were involved in this study. Average age was 74.8 ± 7.2 years. The mean preoperative active range of motion were − 4.1° ± 6.3°in extension and 123.2° ± 15.5° in flexion. All UKAs were performed using fixed bearing type UKA (Zimmer Biomet, ZUK), with adjusting the posterior slope of the proximal tibial bone cut according to the original geometry of the tibia. Routine lateral radiographies of the knee were examined preoperatively, 6 months after the surgery. PTS and knee extension angles with maximal active knee extension (mEXT) and one-leg standing (sEXT) were radiographically measured. We used the fibular shaft axis (FSA) for the sagittal mechanical axis of the tibia. PTS was defined as the angle between the medial tibial plateau and the perpendicular axis of FSA. Extension angles (mEXT and sEXT) were defined as the angles between FSA and distal femoral shaft axis (positive value for hyperextension). The changes of PTS and the influences of PTS on sEXT at each time period were analyzed using simple linear regression analysis (p<0.05).Introduction
Materials & Methods
Acquiring adaptive soft-tissue balance is one of the most important factors in total knee arthroplasty (TKA). However, there have been few reports regarding to alteration of tolerability of varus/valgus stress between before and after TKA. In particular, there is no enough data about mid-flexion stability. Based on these backgrounds, it is hypothesized that alteration of varus/valgus tolerance may influence post-operative results in TKA. The purpose of this study is an investigation of in vivo kinematic analyses of tolerability of varus/valgus stress before and after TKA, comparing to clinical results. A hundred knees of 88 consecutive patients who had knees of osteoarthritis with varus deformity were investigated in this study. All TKAs (Triathlon, Stryker) were performed using computer assisted navigation system. The kinematic parameters of the soft-tissue balance, and amount of coronal relative movement between femur and tibia were obtained by interpreting kinematics, which display graphs throughout the range of motion (ROM) in the navigation system. Femoro-tibial alignments were recorded under the stress of varus and valgus before the procedure and after implantation of all components. In each ROM (0, 30, 60, 90, 120 degrees), the data of coronal relative movement between femur and tibia (tolerability) were analyzed before and after implantation. Furthermore, correlations between tolerability of varus/valgus and clinical improvement revealed by ROM and Knee society score (KSS) were analyzed by logistic regression analysis.Introduction
Materials and Methods
Patients-reported outcome measures (PROMs) have been reported as the important methods to evaluate clinical outcomes in total knee arthroplasty (TKA). The patient satisfaction score in Knee Society Score (KSS-2011) has been used in the recent literatures. Patient satisfaction was subjective parameter, and would be affected by multiple factors including psychological factors and physical conditions at not only affected joint but also elsewhere in the body. The question was raised regarding the consistency of patient satisfaction score in KSS-2011 to other PROMs. The purpose of this study was to investigate the correlation of patient satisfaction in KSS-2011 to other categories in KSS-2011 and to other PROMs including Forgotten Joint Score (FJS-12), EuroQol-5 Dimensions (EQ-5D) and 25-questions in Geriatric Locomotive Function Scale (GLFS-25). 83 patients over 65 years old with osteoarthritic knees were involved in this study. All patients underwent CR-TKAs (Persona CRR). The means and ranges of demographics were as follows: age; 74.5 years old (65–89), Hip-Knee-Ankle (HKA) angle; 12.4 (−6.2–22.5) in varus. We asked patients to fulfill the questionnaire including KSS-2011, FJS-12, EQ-5D and GLFS-25 at 1-year postoperative follow-up visit. KSS-2011 consisted of 4 categories of questions; patient satisfaction (PS), symptoms, patient expectations (PE), functional activities (FA). We evaluated the correlation of PS to other PROMs using simple linear regression analyses (p<0.001).Introduction
Material & Method
The purpose of this study was to evaluate the in vitro effects of apocynin, an inhibitor of nicotinamide adenine dinucleotide phosphate oxidase (NOX) and a downregulator of intracellular reactive oxygen species (ROS), on high glucose-induced oxidative stress on tenocytes. Tenocytes from normal Sprague-Dawley rats were cultured in both control and high-glucose conditions. Apocynin was added at cell seeding, dividing the tenocytes into four groups: the control group; regular glucose with apocynin (RG apo+); high glucose with apocynin (HG apo+); and high glucose without apocynin (HG apo–). Reactive oxygen species production, cell proliferation, apoptosis and messenger RNA (mRNA) expression of NOX1 and 4, and interleukin-6 (IL-6) were determined in vitro.Aims
Methods
The aim of this study was to investigate the effect of hyperglycaemia on oxidative stress markers and inflammatory and matrix gene expression within tendons of normal and diabetic rats and to give insights into the processes involved in tendinopathy. Using tenocytes from normal Sprague-Dawley rats, cultured both in control and high glucose conditions, reactive oxygen species (ROS) production, cell proliferation, messenger RNA (mRNA) expression of NADPH oxidase (NOX) 1 and 4, interleukin-6 (IL-6), matrix metalloproteinase (MMP)-2, tissue inhibitors of matrix metalloproteinase (TIMP)-1 and -2 and type I and III collagens were determined after 48 and 72 hours Objectives
Methods
Both measured resection technique and gap balancing technique have been important surgical concepts in total knee arthroplasty (TKA). Modified gap technique has been reported to be beneficial for the intra-operative soft tissue balancing in posterior-stabilizing (PS) -TKA. On the other hand, we have found joint distraction force changed soft tissue balance measurement and medial knee instability would be more likely with aiming at perfect ligament balance at extension in modified gap technique. The medial knee stability after TKA was reported to essential for post-operative clinical result. We have developed a new surgical concept named as “medial preserving gap technique” for varus type osteoarthritic (OA) knees to preserve medial knee stability and provide quantitative surgical technique using tensor device. The purpose of this study was to compare post-operative knee stability between medial preserving gap technique (MPGT) and measured resection technique (MRT) in PS-TKA. The subjects were 140 patients underwent primary unilateral PS-TKA for varus type OA knees. The surgical technique was MPGT in 70 patients and MRT in 70 patients. There were no significant differences between two groups in the pre-operative clinical features including age, sex, ROM and deformity. Originally developed off-set type tensor device was used to evaluate both center gap and varus angle with 40 lbs. of joint distraction force. The extension gap preparation was identical in both group. In MPGT group, femoral component size and external rotation angle were adjusted depending on the differences of center gaps and varus angles between extension and flexion before posterior femoral condylar osteotomy. The knee stabilities at extension and flexion were assessed by stress radiographies; varus-valgus stress test with extension and stress epicondylar view with flexion, at one-month and one-year after TKA. We measured joint opening distance (mm) at medial and lateral compartment at both knee extension and flexion. Joint opening distances were compared between two groups using unpaired t-test, and the difference between medial and lateral compartment in each group was compared using paired t- test (p<0.05).Introduction
Material & Method
In recent literatures, medial instability after TKA was reported to deteriorate early postoperative pain relief and have negative effects on functional outcome. Furthermore, lateral laxity of the knee is physiological, necessary for medial pivot knee kinematics, and important for postoperative knee flexion angle after cruciate-retaining total knee arthroplasty (CR-TKA). However, the influences of knee stability and laxity on postoperative patient satisfaction after CR-TKA are not clearly described. We hypothesized that postoperative knee stability and ligament balance affected patient satisfaction after CR-TKA. In this study, we investigated the effect of early postoperative ligament balance at extension on one-year postoperative patient satisfaction and ambulatory function in CR-TKAs. Sixty patients with varus osteoarthritis (OA) of the knee underwent CR-TKAs were included in this study. The mean age was 73.6 years old. Preoperative average varus deformity (HKA angle) was 12.5 degrees with long leg standing radiographs. The knee stability and laxity at extension were assessed by stress radiographies; varus-valgus stress X-ray at one-month after operation. We measured joint separation distance (mm) at medial compartment with valgus stress as medial joint opening (MJO), and distance at lateral compartment with varus stress as lateral joint opening (LJO) at knee extension position. To analyze ligament balance; relative lateral laxity comparing to the medial, varus angle was calculated. New Knee Society Score (NKSS) was used to evaluate the patient satisfaction at one-year after TKA. We measured basic ambulatory functions using 3m timed up and go test (TUG) at one-year after surgery. The influences of stability and laxity parameters (MJO, LJO and varus angle at extension) on one-year patient satisfaction and ambulatory function (TUG) was analyzed using single linear regression analysis (p<0.01).Background
Materials & Methods
Knee osteoarthritis (OA) is a major cause of ambulatory disabilities. Although total knee arthroplasty (TKA) has been reported as the most effective treatment for severe knee OA, quantitative evaluation of ambulatory function have not been well investigated. We hypothesized that better functional recovery would result in better patient satisfaction. The purpose of this study was to quantitatively evaluate ambulatory functional recovery and assess the influence on patient satisfaction after TKA. 90 patients (80 females and 10 males) were involved in this study. The mean age at TKA was 75.2 ± 5.8 years. Patients were subjected to 3 meters timed up and go test (TUG) to evaluate ambulatory function. TUG was performed at 6 time periods; before surgery, 2 weeks, 1, 3, 6 months, and 1 year after surgery. We also asked the patients to fill out the questionnaire of patient satisfaction category in the New Knee Society Score at 1 year after TKA. The sequential changes in TUG were analyzed using repeated measures ANOVA (p<0.05). The influences of each parameter (age, TUGs) on the patient satisfaction score were analyzed with simple regression analysis (p<0.05).Background
Material and Methods
This study aimed to examine the effects of SRT1720, a potent SIRT1 activator, on osteoarthritis (OA) progression using an experimental OA model. Osteoarthritis was surgically induced by destabilization of the medial meniscus in eight-week-old C57BL/6 male mice. SRT1720 was administered intraperitoneally twice a week after surgery. Osteoarthritis progression was evaluated histologically using the Osteoarthritis Research Society International (OARSI) score at four, eight, 12 and 16 weeks. The expression of SIRT1, matrix metalloproteinase 13 (MMP-13), a disintegrin and metalloproteinase with thrombospondin motifs-5 (ADAMTS-5), cleaved caspase-3, PARP p85, and acetylated nuclear factor (NF)-κB p65 in cartilage was examined by immunohistochemistry. Synovitis was also evaluated histologically. Primary mouse epiphyseal chondrocytes were treated with SRT1720 in the presence or absence of interleukin 1 beta (IL-1β), and gene expression changes were examined by real-time polymerase chain reaction (PCR).Objectives
Methods
Diabetes mellitus (DM) is known to impair fracture healing. Increasing evidence suggests that some microRNA (miRNA) is involved in the pathophysiology of diabetes and its complications. We hypothesized that the functions of miRNA and changes to their patterns of expression may be implicated in the pathogenesis of impaired fracture healing in DM. Closed transverse fractures were created in the femurs of 116 rats, with half assigned to the DM group and half assigned to the control group. Rats with DM were induced by a single intraperitoneal injection of streptozotocin. At post-fracture days five, seven, 11, 14, 21, and 28, miRNA was extracted from the newly generated tissue at the fracture site. Microarray analysis was performed with miRNA samples from each group on post-fracture days five and 11. For further analysis, real-time polymerase chain reaction (PCR) analysis was performed at each timepoint.Objectives
Methods
The aim of this study was to compare the post-operative radiographic
and clinical outcomes between kinematically and mechanically aligned
total knee arthroplasties (TKAs). A total of 60 TKAs (30 kinematically and 30 mechanically aligned)
were performed in 60 patients with varus osteoarthritis of the knee
using a navigation system. The angles of orientation of the joint
line in relation to the floor, the conventional and true mechanical
axis (tMA) (the line from the centre of the hip to the lowest point
of the calcaneus) were compared, one year post-operatively, on single-leg
and double-leg standing long leg radiographs between the groups.
The range of movement and 2011 Knee Society Scores were also compared
between the groups at that time.Aims
Patients and Methods
Post-operative (postop) lower limb alignment in unicompartmental knee arthroplasty (UKA) has been reported to be an important factor for postop outcomes. Slight under-correction of limb alignment has been recommended to yield a better clinical outcomes than neutral alignment. It is useful if the postop limb alignment can be predicted during surgery, however, little is known about the surgical factors affecting the postop limb alignment in UKA. The purpose of this study was to examine the influence of the medial tibial joint line elevation on postop limb alignment in UKA. Seventy-four consecutive medial UKAs were enrolled in this study. All the patients received a conventional fixed bearing UKA. Pre-operative (preop) and postop limb alignment was examined using long leg radiograph and lower limb alignment changes were calculated. Femoral and tibial osteotomy thickness were measured during surgery. Medial tibial joint line change was defined as polyethylene thickness minus tibial osteotomy thickness and sawblade thickness (1.27mm). Positive values indicated a tibial joint line elevation. Medial femoral joint line change was defined as femoral distal component thickness (6.5mm) minus femoral distal osteotomy thickness and sawblade thickness. Positive values indicated a femoral joint line reduction. Medial joint distraction width was also calculated by tibial joint line elevation plus femoral joint line reduction. The correlation of lower limb alignment change with polyethylene insert thickness, the medial tibial joint line elevation, femoral joint line reduction, or joint distraction width were analyzed.Background
Methods
Recently, tibial insert design of cruciate-substituting (CS) polyethylene insert is employed and widely used. However, in vivo kinematics of using CS polyethylene insert is still unclear. In this study, it is hypothesized that CS polyethylene insert leads to stability of femoro-tibial joint as well as posterior-stabilized (PS) polyethylene insert, even if PCL is sacrificed after TKA. The purpose of this study is an investigation of in vivo kinematics of femoro-tibial joint with use of CS polyethylene insert before and after PCL resction using computer assisted navigation system and tensor device intra-operatively in TKA. Sixty-one consecutive patients who had knees of osteoarthritis with varus deformity were investigated in this study. All TKAs (Triathlon, Stryker) were performed using computer assisted navigation system. During surgery, using a tensor device, after bony cut of femur and tibia, joint gaps were assessed in 0 and 90 degrees in flexion. Then, CS polyethylene tibial trial insert were inserted after trial implantation of femoral and tibial components, before and after resection of PCL, respectively. The kinematic parameters of the soft-tissue balance, and amount of coronal and sagittal relative movement between femur and tibia were obtained by interpreting kinematics, which display tables throughout the range of motion (ROM) in the navigation system. In each ROM (30, 45, 60, 90, max degrees), the data were analyzed with a ANOVA test, and mean values were compared by the multiple comparison test (Turkey HSD test) (p< 0.05).Introduction
Materials and Methods
Range of motion (ROM) is one of the important factor for better functional outcome after total knee arthroplasty (TKA). In posterior cruciate ligament (PCL) retaining (CR) TKA, adequate PCL function is suggested to be important for better kinematics and ROM. However, intraoperative assessment of PCL function is relatively subjective, thus more objective evaluation is required to improve the functional outcomes after TKA. In clinical practice, tibial posterior sagging sign is well known to indicate PCL deficiency. Hence, we hypothesized that intraoperative femorotibial antero-posterior (AP) changes at 90° of flexion indirectly reflected the PCL function and associated with postoperative maximum flexion angles in CR TKA. The purpose of this study was to investigate the correlation between intraoperative femorotibial AP changes at 90° of flexion and postoperative maximum flexion range in navigated CR TKA. Between March 2014 and March 2015, forty patients with varus osteoarthritis underwent primary TKA. All of the cases were using same types of implant (Triathlon; Stryker Orthopedics, Mahwah, NJ, USA), with an image-free navigation system (Stryker 4.0 image-free computer navigation system; Stryker). PCL was retained and cruciate substituting (CS) inserts were used in all cases. The mean age at the time of surgery was 71.7 ± 6.8 years old (ranging: 62 – 85). The mean follow-up was 10.9 ± 6.4 months. After minimum release of medial and lateral soft tissue, resection of anterior cruciate ligaments, and protection of PCL, registration and kinematic measurements were performed prior to bone resection. The kinematic measurements were performed again after implantation. The center of proximal tibial and distal femur were defined during registration. The point of proximal tibia was projected to the mechanical axis of femur and the distance between the projected point and the distal femur at 90° of flexion were measured and defined as femorotibial AP position. Distal relative to the center of distal femur indicates as minus, and proximal relative to the point indicates as plus. The correlation between the intraoperative changes of AP position and postoperative maximum flexion angles were investigated.Introduction
Methods
As the aging society progresses rapidly in Japan, the number of elderly patients underwent TKA is increasing. These elderly patients do not expect to do sports, but regain independency in the activity of daily living. Therefore, we measured basic ambulatory function quantitatively using 3m timed up and go (TUG) test. We clinically experienced patient with medially unstable knee after TKA was more likely to result in the unsatisfactory outcome. We hypothesized that post-operative knee stability influenced ambulatory function recovery after TKA. In this study, we evaluated ambulatory function and knee stability quantitatively, and analyzed the effect of knee stability on the ambulatory function recovery after TKA. Seventy nine patients with varus type osteoarthritic knees underwent TKA were subjected to this study. The mean age of surgery was 72.4 years old. Preoperative standing coronal deformity was 9.6 degrees in varus. TUG test results in less duration with faster ambulatory function. TUG (seconds) was measured at 3 time periods; pre-operatively, at hospital discharge and 1year after surgery. To standardize TUG recovery time during 1 year after TKA, we defined TUG recovery rate as the percentage of recovery time to the pre-operative TUG as shown in the following equation. TUG recovery rate (%) = (TUG pre-op –TUG 1y po) / TUG pre-op ×100 We also evaluated the knee stability at hospital discharge and 1year after surgery. The knee stability at extension and flexion were assessed by varus and valgus stress radiography using Telos (10kg) and stress epicondylar view with 1.5kg weight at the ankle respectively. Image analyzing software was used to measure joint separation distance (mm) at medial as medial joint opening (MJO) and at lateral as lateral joint opening (LJO) at both knee extension and flexion. (Fig.1) The sequential change of TUG was analyzed using repeated measures ANOVA (p<0.05). The influence of joint opening distances (MJO and LJO at extension and flexion) on TUG 1y po and TUG recovery rate were analyzed using simple linear regression analysis (p<0.05).Objective
Materials & Methods
