The aim of this study was to investigate the effect of hyperglycaemia on oxidative stress markers and inflammatory and matrix gene expression within tendons of normal and diabetic rats and to give insights into the processes involved in tendinopathy. Using tenocytes from normal Sprague-Dawley rats, cultured both in control and high glucose conditions, reactive oxygen species (ROS) production, cell proliferation, messenger RNA (mRNA) expression of NADPH oxidase (NOX) 1 and 4, interleukin-6 (IL-6), matrix metalloproteinase (MMP)-2, tissue inhibitors of matrix metalloproteinase (TIMP)-1 and -2 and type I and III collagens were determined after 48 and 72 hours Objectives
Methods
An accelerometer-based portable navigation system (KneeAlign2, OrthAlign Inc., Aliso Viejo, CA) is expected to improve mechanical axis and component alignment compared to conventional instrumentation in total knee arthroplasty (TKA). However, past reports have evaluated its accuracy using only radiographic measurements. The purpose of this study was to analyze the accuracy of the KneeAlign2 system with radiography and more detailed three-dimensional (3D) CT. We targeted 22 patients (24 knees) with severe osteoarthritis who underwent primary TKA using the KneeAlign2 system. Cemented, fixed-bearing, cruciate-retaining prostheses were implanted in all patients. We used postoperative standing-position full-length radiographic evaluation of the lower limbs to measure the hip-knee-ankle angle (HKA), frontal femoral component angle (FFC), and frontal tibial component angle (FTC). However, lower limb rotation and knee flexion could affect radiographic measurement of HKA and the component positioning angle. We used 3D bone models reconstructed from pre- and postoperative CT images to precisely analyze the 3D component positioning. For a 3D matching bone model made from these models, a 2D projection of the pre- and postoperative component positioning planes was made, and the projection angle was measured as angle error compared to the preoperative planned position (Figure 1). Average surgery time and total blood loss on postoperative day 7 were also recorded.INTRODUCTION
METHODS
Dysplastic hip has not only deficiency of acetabulum but also femoral deformity. Therefore, selection of stem is important in cementless THA for dysplasia. Especially using of short stem should be challenge for deformed femur. We studied clinical performance and radiological findings after THA with triple tapered short stem (Optimys hip) for dysplastic hip. From May 2013, we performed cementless THA for osteoarthritis with dysplasia. Seventy-one hips of 67 patients were examined including four males and 63 females. Age at surgery was from 36 to 88 years old (61.7 in average). Surgical approach was used modified Watson-Jones in all hips. Clinical evaluation was used modified Harris hip score (MHSS), incidence of complications and thig pain. Radiological findings were evaluated according Engh's classification and grade of stress shielding. Follow-up period was minimum one year and was 21.3 months in average.Introduction
Materials and method
Triamcinolone acetonide (TA) is widely used for the treatment of rotator cuff injury because of its anti-inflammatory properties. However, TA can also produce deleterious effects such as tendon degeneration or rupture. These harmful effects could be prevented by the addition of platelet-rich plasma (PRP), however, the anti-inflammatory and anti-degenerative effects of the combined use of TA and PRP have not yet been made clear. The objective of this study was to determine how the combination of TA and PRP might influence the inflammation and degeneration of the rotator cuff by examining rotator cuff-derived cells induced by interleukin (IL)-1ß. Rotator cuff-derived cells were seeded under inflammatory stimulation conditions (with serum-free medium with 1 ng/ml IL-1ß for three hours), and then cultured in different media: serum-free (control group), serum-free + TA (0.1mg/ml) (TA group), serum-free + 10% PRP (PRP group), and serum-free + TA (0.1mg/ml) + 10% PRP (TA+PRP group). Cell morphology, cell viability, and expression of inflammatory and degenerative mediators were assessed.Objectives
Methods
Patient matched instrumentation (PMI) have been proposed the accuracy of bone cuts through custom cutting blocks and provide the proper alignment of total knee arthroplasty (TKA). On the other hand, there are some reports that the introduction of PMI for guiding bone cuts could increase the incidence of malalignment in primary TKA. Recent comparisons between patient-specific cutting guides and quantitative assessments of postoperative alignment have revealed the presence of outliers with respect to coronal alignment. The purpose of this study was to assess the implanted component alignment post-operatively between one type of MRI based PMI (Visionaire; Smith & Nephew, Inc, Memphis, Tenn) and conventional surgical instrumentation (CI) using radiographs and CT scan. 32 knees in 32 patients (25 women) with medial type knee osteoarthritis were underwent cruciate retaining TKA between September 2013 and May 2015, and were included in this study. Preoperative MRI scanning of the hip, knee, and ankle was performed for PMI group (n=12) and CT scanning was performed for CI group (n=20) 6 weeks before surgery according to a standard scanning protocol to determine the surgical epicondylar axis (SEA). Postoperatively, we compared operation time, blood loss, and mechanical alignment of two groups. Post-surgical mechanical alignments such as hip-knee-ankle angle (HKA), frontal femoral component angle (FFC), and frontal tibial component angle (FTC) were determined using long leg radiographs (Fig. 1). CT scans were used to assess the condylar twist angle (CTA) made by SEA and posterior condylar axis (Fig. 2). Each measurement was performed by two, blinded independent observers, and interclass correlation for each measurement was calculated. A student's two-tailed t test was used to compare the two cohorts, with statistical significance set at a p-value of <0.05.Introduction
Methods
Fixation patterns of cementless stem were known as proximal or distal part. Distal fixation was seen in fully porous coated stem and stress shielding of the proximal femur was indicative. These phenomena did not lower the clinical results, but technical difficulties were more and more in revision surgery because of infection or dislocation. There was lot of reports that alendronate was effective for treatment of osteoporosis by induction of apoptosis in osteoclasts. We can expect alendronate to modify the bone quality around the stem after cementless THA. We studied prospectively that quantitative computed tomography (QCT) measured bone mineral density around the stem between alendronate group and control. We tried to clarify that stress shielding after cementless THA can be prevented by use of alendronate or not.Introduction
Objectives
To investigate the appropriate dose and interval for the administration
of triamcinolone acetonide (TA) in treating tendinopathy to avoid
adverse effects such as tendon degeneration and rupture. Human rotator cuff-derived cells were cultured using three media:
regular medium (control), regular medium with 0.1 mg/mL of TA (low
TA group), and with 1.0 mg/mL of TA (high TA group). The cell morphology,
apoptosis, and viability were assessed at designated time points.Objectives
Methods
The purpose of the present study was to describe the long-term results of THA for ONFH in patients with SLE. From 1994–2001, 18 cementless THAs (14 SLE patients) were included in the present study. Four hips (3 patients) were lost to follow-up. The remaining 14 hips (11 patients) were available for evaluation. The mean follow-up period was 13.1(range, 10.0–16.4) years. The follow-up rate was 77.8%. The mean age at the time of surgery was 35.2 (range, 27.4–51.0) years.Objectives
Methods
Nociceptive and neuropathic components both contribute to chronic pain. Since these components require different pain management strategies, correct pain diagnosis before and during treatment is important. Freynhagen et al. (2006) reported that they had developed and validated the pain-DETECT questionnaire (PD-Q) to detect neuropathic components in chronic low back pain patients. They also reported that 37% of unselected cohort of chronic LBP patients had predominantly neuropathic pain. However, the extent to which neuropathic components relate to the pathomechanism of pain deriving from osteoarthritis of hip joint remains unknown. The purpose of this study was to utilize PD-Q to investigate the relationship between neuropathic components and pain deriving from osteoarthritis of the hip joint. Between March and August 2010, 125 patients with osteoarthritis of hip joint completed PD-Qs about their pain. From this data set, we investigated whether or not the patients’ pain contained neuropathic components.Introduction
Methods
The purpose of this study was to clarify the incidence of steroid-induced osteonecrosis among different collagen diseases and to evaluate the predictive factors for steroid-induced osteonecrosis in a prospective MRI study. We prospectively used MRI to study 337 eligible collagen disease patients requiring corticosteroid therapy and succeeded in examining 1199 joints (hips and knees) in 302 patients with MRI for at least one year starting immediately after the onset of corticosteroid therapy, a one-year follow-up rate of approximately 90%. The underlying collagen diseases included systemic lupus erythematosus (SLE) in 687 joints and a variety of other collagen diseases in 512 joints.Introduction
Methods
We usually plan surgery for total hip arthroplasty (THA)using pre-operative X-ray templates. However, the technique provides only two dimensional (2D) images and therefore has limitations for planning three-dimensional (3D) objects. Recently it has become possible to describe 3D images using computer programs that use free down loaded computer software (Hip-op) that enable comparison between 3D templates obtained by computed tomography (CT) and 2D conventional X-ray templates. Six hip joints in 6 patients (1 male, 5 females; age range at the time of operation, 49–77 years) were evaluated. Five of the patients suffered from secondary osteoarthritis of the hip (secondary OA), while the remaining patient suffered from osteonecrosis of the femoral head (ONFH). All the patients underwent THA using a cement less femoral stem (ANCA-FIT Wright Medical Technology, Arlington, Tennessee, USA). Pre-operative planning was performed using Hip-op software (Rizzoli Institute, Bologna, Italy). After the THA surgery, we carried out a repeat CT scan that was used to analyze stem fitting in the femur. In all the patients it proved easy to obtain the pre-operative template. In 4 patients, the correct stem size was selected pre-operatively, while in the other 2 patients, the planned stem size was one size smaller than that actually implanted. In the 4 patients in whom the correct stem was selected, 2 had their template correctly assessed by conventional 2D images, 1 patient’s template was one size smaller than the implant, while the remaining patient’s template was two sizes smaller than the implant. In the 2 patients who received implants one size smaller than the CT template, 1 patient had the stem inserted in the virus position while in the other patient a fracture occurred during implantation Preoperative planning for THA using a CT-based computer templating system proved to be a useful technique for the orthopedic surgeons.
