The rotational alignment of the tibia is an as yet unresolved issue for arthroplasty surgeons. Functional variation may be due to minor malrotation of the tibial component. The aim was to find a reliable method for positioning the tibial component in arthroplasty. CT scans of 21 knees were reconstructed in three dimensions and oriented vertically. A plane was taken 20 mm below the tibial spines. The centre of each tibial condyle was calculated from points taken round that condylar cortex. A tibial tubercle centre was also generated as the centre of the circle that best fit points on the surface of the tubercle in the plane of its most prominent point. The derived points were identified by three observers with errors of 0.6 – 1mm. The medial and lateral tibial centres were constant features (radius 24mm ± 3mm, and 22mm ± 3mm respectively). An ‘anatomic’ axis was created perpendicular to a line joining these two points. The tubercle centre was found 20mm ± 7mm lateral to the medial tibial centre. Compared to this axis, an axis perpendicular to the posterior condylar axis was internally rotated by 6° ± 3°. An axis based on the tibial tubercle and the tibial spines was also internally rotated by 6° ± 10°. We conclude that alignment of the knee when based on this ‘anatomic’ axis is more reliable than either of the posterior surfaces. It is also more reliable than any axis involving the tubercle, which is the least reliable feature in the region. The ‘anatomic’ axis can be used in navigated knee arthroplasty for referencing the rotational alignment of the tibial component.