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Orthopaedic Proceedings
Vol. 96-B, Issue SUPP_11 | Pages 37 - 37
1 Jul 2014
EFFECT OF N-ACETYLCISTEINE AND SUBINHIBITORY CONCENTRATION OF ERYTROMYCIN IN COMBINATION WITH ANTIBIOTICS AGAINST STAPHYLOCOCCAL BIOFILMS: A PRELIMINARY STUDY
Molina-Manso D Del-Prado G Lucas-Diaz M Gómez-Barrena E Cordero-Ampuero J Esteban J
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Summary Statement

Combination of antibiotics with N-acetylcisteine and sub-MIC concentration of erythromycin was evaluated in two collection and 16 clinical strains of staphylococci isolated from PJI. The results were strain-dependent, so it evidences the necessity of perform individual studies of biofilm susceptibility.

Objectives

Staphylococci are the most common cause of prosthetic joint infections (PJI) (1), making the treatment of this disease difficult due to the increased resistance to antibiotics of biofilms. Combination between antibiotics and other compounds could be a good alternative. The aim of this study was to evaluate the effect of the combination of two compounds with nine antibiotics in biofilms formed by staphylococcal strains isolated from PJI.

Orthopaedic Proceedings
Vol. 96-B, Issue SUPP_11 | Pages 84 - 84
1 Jul 2014
STAPHYLOCOCCAL BIOFILM FORMATION ON DIAMOND-LIKE CARBON-COATED UHMWPEs: A FIRST APPROACH
Del-Prado G Pascual F Terriza A Molina-Manso D Yubero F Puertolas J Gomez-Barren E Esteban J
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Summary Statement

A study to evaluate biofilm development on different coatings of UHMWPE was performed. We observed a species-specific effect, with S. aureus affected mainly by DLC-F and S. epidermidis by DLC. These data correlates with previous adherence studies.

Introduction

Prosthetic joint infection is intimately related to bacterial biofilms on implant biomaterials. Recently, diamond-like carbon (DLC) coating has been suggested to improve the antibacterial performance of medical grade GUR1050 ultra high molecular weight polyethylene (UHMWPE) supplied by Orthoplastics bacup, UK versus collection and clinical staphylococcal strains. The aim of this study was to make an approximation towards the actual impact of such coatings in biofilm formation.

Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XL | Pages 112 - 112
1 Sep 2012
In Vitro Antibiotic Susceptibility of Staphylococcus Aureus and Staphylococcus Epidermidis Isolated From Retrieved Prosthetic Implants
Molina-Manso D del Prado G Manrubia-Cobo M Ortiz-Pérez A Cordero-Ampuero J Gómez-Barrena E
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INTRODUCTION

Prosthetic joint related-infections (PJRI) are severe complications in orthopaedic surgery. Staphylococcus aureus and Staphylococcus epidermidis are the most commonly isolated pathogens from implants (1). The variable antimicrobial susceptibility found in these microorganisms, makes it necessary to perform individual susceptibility studies in order to select the best antibiotic combination for clinical management (2).

MATERIAL AND METHODS

35 staphylococcal strains (17 S. aureus, 18 S. epidermidis) were isolated from PJRI using a previously described sonication protocol (3). Biofilm-producing collection strains S. aureus 15981 (4) and S. epidermidis ATCC 35984 were also included in the study. In vitro susceptibility was evaluated against 7 antimicrobial agents: rifampin, vancomycin, ciprofloxacin, cotrimoxazole, cloxacillin, clindamycin, and daptomycin. Minimal Inhibitory Concentration (MIC) assays were determined according to EUCAST recommendations and breakpoints (5). Minimal Bactericidal Concentration (MBC) was also calculated by colony counting after plating the well contents.

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