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Orthopaedic Proceedings
Vol. 103-B, Issue SUPP_15 | Pages 23 - 23
1 Dec 2021
EVALUATION OF AN AUTOMATED MULTIPLEX PCR JOINT INFECTION PANEL FOR THE DETECTION/IDENTIFICATION OF PATHOGENS IN 201 SYNOVIAL FLUID SPECIMENS IN A MONOCENTRIC STUDY
Kokenda C Legendre T Abad L Graue C Jay C Ferry T Dupieux-Chabert C kensinger B Laurent F
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Aim

Bone and Joint Infections (BJIs) present with non-specific symptoms and can be caused by a wide variety of bacteria and fungi, including many anaerobes and microorganisms that can be challenging to culture or identify by traditional microbiological methods. Clinicians currently rely primarily on culture to identify the pathogen(s) responsible for infection. The BioFire® FilmArray® Bone and Joint Infection (BJI) Panel (BioFire Diagnostics, Salt Lake City, UT) was designed to detect 15 gram-positive (seven anaerobes), 14 gram-negative bacteria (one anaerobe), two yeast, and eight antimicrobial resistance (AMR) genes from synovial fluid specimens in an hour. The objective of this study was to evaluate the performance of an Investigational Use Only (IUO) version of the BioFire BJI Panel (BBJIP) compared to conventional used as reference methods.

Method

In a monocentric study, leftover synovial fluid specimens were collected in a single institution including 4 hospitals and tested using conventional bacterial culture (Standard of Care (SoC)) according to routine procedures following French national recommendations. Specimen has been placed in a refrigerator (4°C) as soon as possible after collection and stored for less than or equal to 7 days before enrollment. Performance of the IUO version of the BBJIP was determined by comparison to SoC for species identification.

Orthopaedic Proceedings
Vol. 100-B, Issue SUPP_17 | Pages 68 - 68
1 Dec 2018
STAPHYLOCOCCUS AUREUS BONE AND JOINT INFECTION: COMPARISON OF RIFAMYCIN INTRAOSTEOBLASTIC ACTIVITY AND IMPACT ON INTRACELLULAR EMERGENCE OF SMALL COLONY VARIANTS
Abad L Diot A Josse J Tasse J Lustig S Ferry T Laurent F Valour F
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Aim

Leading etiology of Bone and Join infections (BJI), Staphylococcus aureus (SA) is responsible for difficult-to-treat infections mainly because of three persistence factors: (i) biofilm formation, (ii) persistence within bone cells and (iii) switch to the small colony variant (SCV) phenotype. The impact of rifampin on these mechanisms gave it a prominent place in orthopedic device-associated BJI. However, resistance emergence, intolerance and drug interactions cause significant concerns. In this context, other rifamycins – namely rifapentine and rifabutin – have poorly been evaluated, particularly toward their ability to eradicate biofilm-embedded and intracellular reservoirs of SA.

Method

This study aimed at comparing the intracellular activities of and SCV induction by rifampin, rifabutin and rifapentine in an in vitro model of osteoblast infection. Four concentrations were tested (0.1xMIC, MIC, 10xMIC, 100xMIC) against three SA strains (6850 and two clinical isolates involved in recurrent BJI)