On this basis we would recommend that all patients have the biopsy site marked at the time of biopsy and a further audit will be carried out to evaluate this change in practice.
Complications developed in seven patients: two implants failed requiring revision, one peri-prosthetic fracture occurred, one developed a flexion deformity of 25 degrees at the knee joint, which was subsequently overcome and three died of disseminated disease.
The five most common histopathological diagnoses were chondrosarcoma (9%), osteosarcoma (9%), meta-static renal carcinoma (8%), giant-cell tumour (6%), lymphoma (5%). 77% of biopsies yielded a tissue diagnosis. The remaining 23% underwent open biopsy, repeat image-guided needle biopsy or were not further investigated. In the 30 cases (23%) of non-diagnostic biopsies 80% of these lesions had no extra-osseous component to them and the remaining 20% had a very small extra-osseous component.
Our aim was to review the short-term clinical results of a single-institution cohort undergoing opening wedge high tibial osteotomy (HTO). We undertook a prospective clinical and radiographic review of our cohort of patients who had undergone opening wedge HTO for varus malalignment. The Cincinnati scoring system was used for objective assessment. Pre- and post-operative radiographs were evaluated and Blackburne-Peel (BP) and Insall-Salvati (IS) ratios recorded, as well as integrity of the lateral hinge. We reviewed 55 knees (51 patients: 34 men and 17 women; mean age, 44.2years; range 34–58years) followed up for a minimum 12 months (range, 12–62months). All patients had relief of pain, but six met our criteria of failed treatment where either revision fixation was required or proceeded to total knee arthroplasty for persisting symptoms. Cincinnati scores were 94.5% excellent (52/55) and 5.5% good (3/55) at 1 year, whilst at last follow-up they were 87.2% excellent (48/55), 9.1% good (5/55) and 3.6% fair (2/55). There was a significant improvement in mean American Knee Society score at 1 year and maintained at last follow-up (p<
0.05). Radiographically the lateral hinge was noted to be breached in 9.1% (5/55), but no incidence of non-union was identified. There was no significant change in IS index, however BP index diminished by a mean 15.3% (range, 7.4–28.2%). Medial opening wedge tibial osteotomy results in patellar infera, but successful clinical and functional outcomes have been demonstrated. The fact ther e is inconsistency between the two indices assessing patellar height ratio we believe reflects the inherent variability in the techniques employed. Distalisation of the tibial tubercle will mean the IS ratio remains unaffected, whilst the BP index more accurately demonstrates the lowering of patella relative to the joint line. However there may be other factors which are not immediately appreciated, such as changes in the tibial inclination or antero-posterior translation.
We retrospectively studied 67 patients who underwent proximal humeral replacement with the Bayley-Walker prosthesis, for tumour of the proximal humerus between 1997 and 2007. Of the 67 patients 10 were lost to follow up. Of the 41 surviving patients, function was assessed using the Musculoskeletal Tumour Society (MSTS) Score and the Toronto Extremity Salvage Score (TESS) questionnaire. 4 of the 41 patients received the new Bayley-Walker ‘captured’ proximal humeral replacement. The mean age was 46 years (7–87). The mean MSTS score at follow-up was 72.0 % and the mean TESS score was 77.2 %. The sub-group of 4 pts who received the new captured prosthesis had a mean MSTS score of 77.7 %. There was no mechanical failure of any prostheses in the follow up period. Endoprosthetic replacement for tumour of the proximal humerus with the Bayley-Walker prosthesis, is a reliable operation yielding reasonable functional results and good long-term prosthesis survivorship. The performance of this prosthesis is expected to improve further with the new ‘captured’ prosthesis.
Our aim was to review the short-term clinical results of a single-institution cohort undergoing opening wedge high tibial osteotomy (HTO). We undertook a prospective clinical and radiographic review of our cohort of patients who had undergone opening wedge HTO for varus malalignment. The Cincinnati scoring system was used for objective assessment. Pre- and post-operative radiographs were evaluated and Blackburne-Peel (BP) and Insall-Salvati (IS) ratios recorded, as well as integrity of the lateral hinge. We reviewed 55 knees (51 patients: 34 men and 17 women; mean age, 44.2years; range 34–58years) followed up for a minimum 12 months (range, 12–62months). All patients had relief of pain, but six met our criteria of failed treatment where either revision fixation was required or proceeded to total knee arthroplasty for persisting symptoms. Cincinnati scores were 94.5% excellent (52/55) and 5.5% good (3/55) at 1 year, whilst at last follow-up they were 87.2% excellent (48/55), 9.1% good (5/55) and 3.6% fair (2/55). There was a significant improvement in mean American Knee Society score at 1 year and maintained at last follow-up (p<
0.05). Radiographically the lateral hinge was noted to be breached in 9.1% (5/55), but no incidence of non-union was identified. There was no significant change in IS index, however BP index diminished by a mean 15.3% (range, 7.4–28.2%). Opening wedge HTO provides a means of relieving stress distribution through the medial tibiofemoral compartment and results in effective relief of symptoms with improvement in functional outcome and quality of life.
38 patients (41 knees) who received a primary SMILES knee prosthesis by one of the senior authors between 1990 and 2006 were retrospectively studied. Knee function was assessed pre and post-operatively using the Oxford knee score (0–48 scoring system) and the Knee Society Score. Patients receiving surgery for tumours were excluded. The main indications for primary SMILES were bone loss and ligamentous laxity.
2 patients died and 5 were lost to follow-up. 2 patients required revision surgery (one for infection and one for re-bushing). Post-operative complications included peroneal nerve palsy (1) and DVT (1). The mean Oxford knee score improved from 9 pre-op to 44 post-op, and the mean knee society score improved from 24 pre-op to 71 post-op. The average range of motion was 57 degrees pre-op and 88 degrees post-op.
1) observational study to analyse surgeon’s and anaesthetist’s attitude to transfusion 2) prospective study to analyse the influence of perioperative haemoglobin concentration (Hb) on post-operative fatigue, hand grip strength, duration of in-patient physiotherapy and post-operative morbidity score (POMS) and also the prognostic factors to predict functional recovery.
200 patients (88 THR, 99 TKR, 13 hip resurfacing) were evaluated. Blood Hb, hand grip strength and vigour scores using fatigue questionnaire were estimated both preoperatively and at 3 days following surgery. POMS and the required duration of in-patient physiotherapy were also noted. The protocol for blood transfusion was for those with Hb less than 8 g/dL and/or post-operative symptoms attributable to anaemia.
A greater fall in postoperative Hb correlated significantly with a greater reduction in post-operative vigour score (p=0.02). Also a greater fall in vigour score was found to correlate significantly with the duration of in-patient physiotherapy (p<
0.001). A reduction in Hb of >
4g/dL from the pre-operative Hb predicted a significantly higher reduction in vigour score (p=0.03). A weak correlation was seen between a fall in Hb and POMS (p=0.09). A higher pre-operative Hb did not reduce the required duration of in-patient physiotherapy (p=0.72). There was no correlation between post-operative Hb and POMS (p=0.21) or duration of in-patient physiotherapy (p=0.20). A higher pre-operative grip strength predicted an early date of discharge by the physiotherapists (p=0.02).
The mean clinical follow-up was 39.4 months (13mths to 8 years) and the mean timing of biopsy was 14.8 months. The mean age at the time of surgery was 32 years (15 to 55 years). The site of defect was as follows: medial femoral condyle-95, lateral femoral condyle-25, trochlea-7, patella 27 and multiple sites- 12. The mean proportion of viable cells available for implantation was 96.3 % (range: 86 to 100) and the mean number of multiplication of cells during culture was 90 (range: 9 to 667).
The most favourable sites were lateral femoral condyle and trochlea where as the least favourable site was patella. There was no correlation between the mental score of patients and the final clinical result. Improvement in functional score was significantly higher among those who had a higher pre-operative function (p<
0.001). There were 7 patients who had previously failed micro-fracture and all of them obtained significant improvements in pain and function. Those who had a higher proportion of viable cells after cell culture demonstrated a tendency towards better outcome, but failed to reach statistical significance (p=0.14). There was no correlation between the number of cell multiplications at the time of cell culture and final clinical outcome (p=0.65). There was no significant difference in clinical outcome between the ACI- C and MACI techniques of ACI (p>
0.05).
We aimed to determine whether proximal femoral bone remodelling, as measured by DEXA scan for each Gruen Zone, differed for 3 different femoral stem designs. These were:
Group 1 – Triple taper polished (C-stem J&
J DePuy) Group 2 – Double taper polished (TPS J&
J DePuy) Group 3 – Collared satin finish stem (Stanmore Biomet) Ethical Committee approval was obtained and all patients gave informed consent. All operations were performed by 3 surgeons using a standard lateral approach, third generation cementing technique and a standard post-operative regime. Seventy-five patients were randomised prospectively using a stratified randomisation programme for known risk factors in bone density. The randomisation was stratified by age, sex, Charnley Group (A or B), BMI and femoral neck bone mineral density. There were 25 patients in each Group, 5 patients did not have complete data sets and were therefore withdrawn from evaluation. The Groups were similar with regard to age, sex, BMI, BMD and Charnley Group. Patients were followed up at 3, 6, 12 and 24 months by Nurse Practitioners for clinical and radiological evaluation and DEXA scan at each time of follow-up. There were no catastrophic failures of sepsis or dislocation. All patients functioned well at 24 months by Harris Hip Score. The bone remodelling pattern will be described for each stem design. Findings are summarised thus. In the lateral Gruen Zones the triple taper and collared stem design faired better and both behaved differently to the double taper design. In Gruen Zone 7, all stems demonstrated reduced bone density as compared to pre-operative scans but this was less with the triple taper design.
46 Sacral chordoma patients treated between 1987 and 2004 are reviewed. The importance of early diagnosis, adequate surgical margin and post operative radiotherapy for optimum outcome and survival is stressed. There were 33 male and 13 female patients, with a mean age of 61 years (38–73 years). The surgical approach depended on the level and extent of the lesion, with an anteroposterior approach used in 23 and posterior approach in 17 patients. 20 had partial sacrectomy, 17 had subtotal sacrectomy and 3 underwent total sacrectomy. 6 patients were deemed inoperable and received palliative therapy. 14 patients received radiotherapy post-operatively. The length of average follow up was 4.27 years (range 2–15.7 years). Low back pain was the most common presenting symptom (80%), and 50% patients had a palpable mass. The mean duration of symptoms prior to diagnosis was 2 years (range 1 month–10 years). Examination revealed a palpable mass in 7 both externally and on rectal examination. 10 had a palpable mass on rectal examination but not externally. 2 patients presented with multiple metastases and another 2 with widespread local disease. Excision was complete in 23 patients and incomplete in 17. Histology revealed dedifferentiation in 4. Complete excision margin was achieved in 69.6% through combined approach and 52.9% through posterior approach only. 24 patients (52%) had local recurrence. Without adjuvant radiotherapy the mean disease free period following complete excision was 3.5 years, compared to 0.9 years following incomplete excision. Adjuvant radiotherapy extended the mean disease free period following incomplete excision to 1.8 years. The authors conclude that an early diagnosis and careful examination is important. Wide excision remains the mainstay of treatment. If excision is incomplete radiotherapy increases the disease free period although local recurrence is inevitable. The use of a combined approach increases the likelihood of complete excision.
the influence of histology on durability of cartilage repair following collagen-covered autologous chon-drocyte implantation (ACI-C) in the knee. the relationship between macroscopic grading and durability of cartilage repair; and the relationship between macroscopic appearance and histology of repair tissue.
There was no correlation between the ICRS grading and MCRS either at one year (p=0.12) or at the latest follow-up (p=0.16). Also, the ICRS grading of the repair tissue did not correlate with its histological type (p=0.12).
Excision was complete in 23 patients and incomplete in 17. Histology revealed dedifferentiation in 4. Complete excision margin was achieved in 69.6% through combined approach and 52.9% through posterior approach only. 24 patients (52%) had local recurrence. Without adjuvant radiotherapy the mean disease-free period following complete excision was 3.5 years, compared to 0.9 years following incomplete excision. Adjuvant radiotherapy extended the mean disease-free period following incomplete excision to 1.8 years.
The modified Cincinnati scores (MCRS) of eighty-six patients were evaluated prospectively at one year and at the latest follow-up following ACI-C (mean follow-up= 4.7 years. Range= 4 to 7 years). All these patients underwent biopsies of their cartilage repair site performed at variable periods between six months and five years following ACI-C (mean=22.2 months). The neo-cartilage was graded as hyaline (n=32), mixed fibrohyaline (n=19), fibrocartilagenous (n=35) and fibrous (n=0).
However, their clinical scores at the latest follow-up demonstrated a significantly superior result for those with hyaline repair tissue when compared to those with mixed fibro-hyaline and fibro-cartilagenous repair tissue (p=0.05). The percentage of patients with excellent and good results for those with hyaline, mixed fibro-hyaline and fibro-cartilagenous repair was 75, 42 and 68.6 respectively. Their mean MCRS were 70.6, 56.8 and 63.9 respectively.
6 prostheses that used ball bearings to achieve length (designed in 1981) were implanted, 3 (50 %) were revised due to mechanical failure. 19 prostheses that utilised external C-washes (1998) to achieve length were implanted, 6 (32 %) were revised, half of these for mechanical failure. Of the 98 minimally invasive prostheses (1992) that utilized an Allan key and screw-jack mechanism to lengthen, 14 (14 %) were revised, half of these for infection. 17 non-invasive extendable endoprostheses (2001) that are lengthened by electromagnetic coupling have been implanted so far. There has only been 1 (6 %) revision. This was due to full extension being reached.
Key Words: Bone tumour, children, endoprosthesis, survivorship.
A higher pre-operative Hb did not reduce the required duration of in-patient physiotherapy (p=0.72). There was no correlation between post-operative Hb and POMS (p=0.21) or required duration of in-patient physiotherapy (p=0.20). A higher pre-operative grip strength predicted an early date of discharge by the physiotherapists (p=0.02).
Only patients with severe preoperative knee function showed deterioration in outcome measures from three years, all other patients maintained improvements.
Visual Analogue Score and Bentley Functional rating score showed significant improvements compared to pre-operative levels (p<
0.0001) with ongoing yearly sequential improvement. Patient Rating and Brittberg scores, both subjective patient scores, similarly showed continuing improvements in the years following surgery.
Introduction: The distal tibia is an uncommon site for primary malignant bone tumours and the treatment of choice for most patients is a below knee amputation. Patients who decline an amputation may be offered an endoprosthetic replacement. This is a technically challenging operation and may be associated with high morbidity.
Bone grafts are frequently used to augment bone healing. Autologous bone graft is the gold standard for osteogenesis but is limited by availability and donor site morbidity. The processing required to lower the immunogenicity of allograft also reduces the osteogeneic properties. Bone marrow contains mesenchymal stem cells (MSCs) which differentiate into osteoblasts, forming bone. Our study examined the use of bone marrow to enhance the osteogenic properties of allograft. Bioactive proteins within allogenic bone graft stimulate marrow-derived MSCs to differentiate into osteoblasts, thereby increasing the osteogenic nature of the graft. After informed consent, bone marrow aspirates were taken from five patients during orthopaedic operations. Freeze-dried ethylene oxide treated allograft, from a number of donors, was obtained from the bone bank. MSCs isolated from each marrow aspirate were grown on eight samples of test allograft. Further allograft was heated to 70°C to denature the osteogenic proteins and MSCs from each aspirate were grown on 8 samples, as a negative control. Osteoblastic differentiation of MSCs cultured on the types of allograft was compared. Scanning electron microscopy confirmed that MSCs covered the allograft after 14 days. Transmission electron microscopy showed that cells on the test allograft were characteristic of osteoblasts and produced collagen extracellular matrix. The levels of osteoblastic proteins, ALP, osteopontin and Type I pro-collagen, produced by cells on test allograft were significantly greater compared with heat-treated control (P<
0.005), after days 7 and 14. Our study showed that marrow-isolated MSCs could be successfully cultured on allograft. As the levels of osteoblastic proteins increased significantly when MSCs were grown on allograft, osteogenic proteins within allograft caused MSCs to change into osteoblasts. This confirms that autologous marrow MSCs could be grown on allograft to increase its osteogenic prior to grafting, resulting in increased rate of bony healing.
Endoprosthetic reconstruction as a form of limb salvage in the management of malignant disease is common. We present our experience with custom-made distal femoral replacement as a form of limb salvage in the absence of malignancy. 49 cases of distal femoral replacement were identified using the unit database. There were 18 males and 31 females, with a mean age at operation of 62.3 years (range 26–86). There were 29 cases of failed total knee replacements, 8 cases of fracture associated with bone loss, non-union or deformity, 7 periprosthetic fractures, 3 aneurysmal bone cysts, and one case each of avascular necrosis and Gorham’s disease. Clinical and radiographic review of all available patients, including a functional assessment with the Musculoskeletal Tumour Society (MSTS) Score and Toronto Extremity Salvage (TES) score was undertaken. The average follow-up was 5.4 years (range 1 to 29 years). Three types of endoprosthesis were used, rotating hinge, fixed hinge and arthrodesis prosthesis. One patient required amputation at 2 months following post-operative wound infection with methicillin-resistant staphylococcus aureus and subsequent inability to provide adequate soft tissue coverage. There was one revision at 16 months for deep prosthetic infection. 21 patients were available for functional follow-up. The mean MSTS score was 63.7 (range 16.0–86.7) and the mean TES score was 59.4 (range 9.4–87.5). Custom-made distal femoral replacements have an established role in limb-salvage surgery for malignant disease. This series demonstrates the reconstructive capability of custom prostheses in non-malignant disease, where deformity causes functional embarrassment or when massive bone loss would normally lead to amputation.
The purpose of this study was to determine whether the low rate of mechanical loosening of the SMILES rotating hinge distal femoral endoprosthesis relates to the hydroxyapatite (HA)-coated, grooved collar of the femoral component. A database was used to identify two groups of cases of primary distal femoral replacement with a custom-designed and manufactured SMILES endoprosthesis at our unit; those with the collared femoral component (“collar group”), and those without a collar (“non-collargroup”). From these two groups, patients were pair-matched for age and length of bone resection. A retrospective review of serial biplanar pairs of radiographs of each patient, assessing radiolucent lines and extracortical bone pedicle. 11 matched pairs were identified, (14 females, 8 males), with a mean age of 36 years, (range 16–66). The pathology was primary bone tumour in 20 cases, (17 malignant, 3 benign), and metastatic disease in 2 cases. Mean length of follow-up was 85 months, (range 27–122). Radiolucent line score (RLS) progression over time was significantly lower in the collar group, (0.01 vs 0.73, p=0.001) (fig. 1 &
2), as was the mean final RLS, (2.72 vs 7.81, p=0.02). Mean RLS per radiographic quadrant was 0.56 in cases in which a bony pedicle was ingrown onto the prosthesis, (exclusively in the collared-group), 2.41 in cases in which the pedicle was not ingrown, (most prevalent in the non-collared group), and 1.02 in those cases without any pedicle formation, (ANOVA analysis, p=0.0002). This study demonstrates that the HA-coated, grooved collar significantly reduces the progression of radiolucent lines, and consequently the overall RLS, explaining the reduced rate of mechanical loosening of the collared endoprosthesis. A bony pedicle that does not incorporate onto the prosthesis surface may be associated with an increase in radiographic loosening.
The aim of this study was to compare the accuracy of image guided (ultrasound or CT) percutaneous needle biopsy to percutaneous needle biopsy without image guidance in diagnosis of soft tissue tumours. Eighty-eight consecutive patients with soft tissue lesion who were referred to the soft tissue tumour unit underwent percutaneous needle biopsies of their lesion either with image guidance or without. Sixty-one out of these 88 patients subsequently underwent excision of their lesion and the excised specimen was then subjected to histological examination. The accuracy of image guided percutaneous needle biopsy and percutaneous needle biopsy without image was then calculated by comparing the histological results of the needle biopsy to that of excision biopsy. The diagnosis accuracy of image guided percutaneous needle biopsy was 92% (34 out 37) compared to 79% (22 out of 28) for percutaneous needle biopsy without image. In 3 out of the 28 patients who had percutaneous needle biopsy without image guidance, there was insufficient material obtained from the needle biopsy to allow a histological diagnosis. This was not the case with any of the patients who had image guided percutaneous needle biopsy.
This prospective study analyses the histological results of autologous chondrocyte transplantation in patients with articular cartilage defects of the knee joint. Chondrocytes from a non-weight bearing area of the knee were harvested and then cultured in vitro. Re-implantation involved injection of the chondrocytes into the defect, which was then sealed with a collagen membrane. One year post-op, patients were evaluated by clinical, arthroscopic and histological assessment. A biopsy of the transplanted region was examined by staining with Erlich’s H&
E and Safranin 0, polarised light microscopy and by analysis with S100 and immunohistochemistry. Hyaline cartilage content was further assessed by examination of Type IIa &
lIb collagen mRNA expression using in-situ hybridisation. The median age was 31 years. 63 knees were treated. Solitary lesions were treated in 61 knees with two defects being treated in three knees (66 defects in total). The defects were located on the medial femoral condyle in 39 cases, lateral femoral condyle in 14, trochlea in 2 and patella in 11. The defect size ranged from 1–7 cm2 (mean area 3cm2 ). 40 patients had at least one-year follow-up. Using the Brittberg Rating, 11 had excellent results, with 15 good, 10 fair and 4 poor. The mean Lysholm and Gillquist scores improved from 44 pre-op to 77 one-year post-op. Biopsy at one year conftrmed the presence of hyaline cartilage in 22 out of 32 cases (69%). In-situ hybridisation confirmed the presence of Collagen type II in the deep zones of the biopsy with a fibrocartilaginous appearance superficially.
The purpose of this study was to evaluate the results of custom-made endoprosthetic reconstruction for both tumourous and non-tumourous conditions around the elbow joint. 28 consecutive cases of endoprosthetic elbow reconstruction, performed between 1989 and 2003, were identified using the unit database. There were 12 males and 16 females, with a mean age 48.9 years, (range 14–84). There were 16 cases of malignant tumour (10 primary, 6 secondary), 3 cases of benign tumour (one each of giant cell tumour, osteoblastoma and pigmented villonodular synovitis) and 10 non-oncological cases, (5 cases of fracture, 3 failed total elbow replacements and 1 infected synostosis). A distal humeral prosthesis was used in 16 patients, distal humeral and proximal ulna in 10 patients, proximal ulna with a humeral component in one patient and proximal radial replacement in one patient. Clinical and radiographic review of all available patients, including a functional assessment with The Toronto Extremity Salvage score (TESS) was undertaken. 28 consecutive cases of endoprosthetic elbow reconstruction, performed between 1989 and 2003, were identified using the unit database. There were 12 males and 16 females, with a mean age 48.9 years, (range 14–84). There were 16 cases of malignant tumour (10 primary, 6 secondary), 3 cases of benign tumour (one each of giant cell tumour, osteoblastoma and pigmented villonodular synovitis) and 10 non-oncological cases, (5 cases of fracture, 3 failed total elbow replacements and 1 infected synostosis). A distal humeral prosthesis was used in 16 patients, distal humeral and proximal ulna in 10 patients, proximal ulna with a humeral component in one patient and proximal radial replacement in one patient. Clinical and radiographic review of all available patients, including a functional assessment with The Toronto Extremity Salvage score (TESS) was undertaken. Endoprosthetic reconstruction around the elbow joint is effective in a wide range of pathologies, allowing in most cases a reasonable level of function, even following two-stage revision for infection.
Five of the six patients were treated with surgical excision.
The number of cases that we have seen in a short time may also be an indication that this syndrome is not as rare as scarcity of the published cases would imply.
Soft tissue balance is known to be an important factor for the success of Total Knee Arthroplasty (TKA). Traditional surgical techniques involve soft tissue releases and bony cuts to achieve the correct balance. Evaluation of balance is currently based on subjective intraoperative clinical assessment, or the “feel” of the knee. More recently, an instrument to objectively measure soft tissue balance following bony cuts has been developed. Soft tissues releases using this instrument may be extensive.
Clinical outcomes during the first two postoperative years did not vary according to repair morphology type, though hyaline repair was associated with better clinical outcomes beyond 2 years; At 1 year, good to excellent clinical scores were observed in 29 (78.4%) patients with hyaline-like repair, 23 (76.7%) patients with fibrohyaline repair, and 54 (74.0%) patients with fibrocartilage repair. By years 3 and 4 post-implantation, clinical scores further improved in patients with hyaline-like repair yet declined in those with fibrocartilage and fibrohyaline. The difference was significant at 3 years though not at 4 due to the small number of cases.
The purpose of this study is to demonstrate that definitive surgery (extraperiosteal excision) is required in patients with osteofibrous dysplasia (OFD) due to the risk of recurrence and co-existent adamantinoma OFD is an unusual childhood condition, which almost exclusively affects the tibia. It is thought to follow a slowly progressive course and to stabilise after skeletal maturity. The possible link with adamantinoma is controversial with some authors believing that they are part of one histological process. This therefore provides difficulty in recommending treatment options A retrospective review of OFD was conducted. Using the Stanmore Bone Tumour Unit database 22 cases were identified who were initially diagnosed with OFD or were diagnosed on final histology. All cases were tibial except one lesion in the ulna and one in the fibula Management was diverse depending on the severity of symptoms and the extent of the lesions encountered. Definitive (extraperiosteal) surgery in the majority of our patients was localized excision for small lesions (less than 50% of the bony circumference) and segmental excision followed by reconstructive surgery for more extensive ones. Seven patients had a sharkbite excision and a further seven were treated with fibula autografting. Of the latter group, one required further excision and bone transport due to recurrence of OFD. An additional five underwent bone transport &
distraction osteogenesis using the Ilizarov technique and one had a proximal tibial replacement. Nine initially underwent curettage, but eight recurred (recurrence rate 88.9%). No recurrences occurred following localized extraperiosteal excisions and bone transport. There were three confirmed cases of adamantinoma. In view of the risk of association of OFD with adamantinoma, and to some extent the continuous morbidity of OFD if left untreated, we believe that radical extraperiosteal excision is indicated in most if not all cases of OFD
To tissue-engineer bone suitable cells need to be grown on a scaffold. In this study we grew human marrow cells as they can differentiate into osteoblasts, on porous hydroxyapatite (HA) scaffolds, as this is osteoconductive, allows cell penetration and in growth of capillaries after implantation. Increased extravascular perfusion through bone increases new bone formation. So we reproduced these physiological conditions in our novel bioreactor by perfusing scaffolds at 6ml/hr.
After 14 days in bioreactor culture the HA was covered with cuboidal cells, consistent with osteoblasts, however in static culture cells remained fibroblastic. TEM results showed that MSCs in the bioreactor produced organised collagen matrix after 21 days and osteoid by 28 days, but no collagen matrix was observed following static culture. ALP and PICP were significantly greater over 15 days culture when in our bioreactor.
Moreover, MSCs grown on HA in the bioreactor produced significantly more ALP and PICP indicating osteoblastic differentiation. Furthermore, bone osteoid was produced. Therefore this culture method could be use to convert autologous MSCs from human marrow into tissue-engineered bone which could be used to heal defects after tumor excision.
We prospectively randomised 104 consecutive patients undergoing primary cemented total knee arthroplasty to receive either a standard suction drain© (Redivac) or autologous transfusion drain® (Bellovac). There were fifty two patients in each group. Randomisation was performed using a software program (Minim) which set to stratify patients based on their age, sex and body mass index (BMI). All procedures were performed under pneumatic tourniquet. Drains were released in recovery room 20 minutes after surgery and were removed 24 hours following surgery. Blood collected in the standard suction drain was discarded but blood collected in the autologous transfusion drains was transfused unwashed to the patient within six hours of collection. 13 patients (25%) in the study group had two or more units of homologous blood transfused in addition to the blood collected postoperatively and re-transfused (Average= 438mls). 12 patients (23%) in the control group had two or more units of homologous blood transfused. No sepsis, transfusion reactions, or coagulopathies were associated with the autologous blood re-transfused in the study group. The use of autologous transfusion system (Bellovac) proved to be safe but failed to reduce the need for postoperative homologous blood transfusion following uncomplicated total knee arthroplasty.